The existence of separate peroxisomal pools of free coenzyme a and long-chain acyl-CoA in rat liver, demonstrated by a specific high performance liquid chromatography method.

1. Tiadenol administration of rats lead to an increased hepatic content of unesterified and esterified CoA. 2. Liver homogenates from normal tiadenol treated rats were fractionated by differential centrifugation and fractions enriched in peroxisomes were subfractionated by isopycnic density gradient centrifugation. 3. The analysis demonstrated that purified peroxisomes contained a separate pool of free CoASH and long-chain acyl-CoA. 4. The data also provides indications of the presence of palmitoyl-CoA synthetase in the peroxisomes.