Literature DB >> 6822146

Determination of linear fluorescence intensities from flow cytometric data accumulated with logarithmic amplifiers.

K A Muirhead, T C Schmitt, A R Muirhead.   

Abstract

Logarithmic amplifiers are useful in accumulating flow cytometric data with a large dynamic range. However, quantitative comparison of fluorescence intensities for different samples or different subpopulations within a sample is simplified by the conversion of data from log space back to linear space. A method is described in which fluorescent polystyrene spheres of differing intensities are used to construct a calibration curve for the logarithmic intensity scale. This allows calculation of relative linear intensity for each channel of the logarithmically accumulated data and determination of linear fluorescence means and coefficients of variation for comparative purposes. Fluorescent spheres of appropriate intensity may also be used as internal standards to monitor instrument and/or stain stability for samples accumulated using logarithmic amplifiers.

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Year:  1983        PMID： 6822146     DOI： 10.1002/cyto.990030404

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  17 in total

Review 1.  Membrane Ig-mediated triggering of B cell tolerance and B cell clonal expansion: implications for rheumatoid factor production in rheumatoid synovitis.

Authors:  P K Mongini; S M Rudich
Journal:  Springer Semin Immunopathol       Date:  1989

2.  The cellular basis for lack of antibody response to hepatitis B vaccine in humans.

Authors:  E Egea; A Iglesias; M Salazar; C Morimoto; M S Kruskall; Z Awdeh; S F Schlossman; C A Alper; E J Yunis
Journal:  J Exp Med       Date:  1991-03-01       Impact factor: 14.307

3.  Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types.

Authors:  V Band; R Sager
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

4.  Expression of urokinase plasminogen activator receptor on monocytes from patients with relapsing-remitting multiple sclerosis: effect of glatiramer acetate (copolymer 1).

Authors:  R Balabanov; D Lisak; T Beaumont; R P Lisak; P Dore-Duffy
Journal:  Clin Diagn Lab Immunol       Date:  2001-11

5.  Recognition of HLA-A2 and -B7 antigens by cloned cytotoxic T lymphocytes after gene transfer into human and monkey, but not mouse, cells.

Authors:  J A Barbosa; S J Mentzer; G Minowada; J L Strominger; S J Burakoff; P A Biro
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

6.  Differential expression of Ia molecules by human monocytes.

Authors:  T A Gonwa; J D Stobo
Journal:  J Clin Invest       Date:  1984-09       Impact factor: 14.808

7.  Expression of a granule membrane marker on the surface of neutrophils permeabilized with digitonin. Correlations with Ca2+-induced degranulation.

Authors:  J E Smolen; R F Todd; L A Boxer
Journal:  Am J Pathol       Date:  1986-08       Impact factor: 4.307

8.  Flow Cytometric Analysis of Characteristics of Hybridization of Species-Specific Fluorescent Oligonucleotide Probes to rRNA of Marine Nanoflagellates.

Authors:  J Rice; M A Sleigh; P H Burkill; G A Tarran; C D O'connor; M V Zubkov
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

9.  Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway.

Authors:  S C Meuer; R E Hussey; D A Cantrell; J C Hodgdon; S F Schlossman; K A Smith; E L Reinherz
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

10.  Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mo1 deficiency. Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes.

Authors:  M A Arnaout; H Spits; C Terhorst; J Pitt; R F Todd
Journal:  J Clin Invest       Date:  1984-10       Impact factor: 14.808
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