| Literature DB >> 6821395 |
D E Yelton, C Desaymard, M D Scharff.
Abstract
Rat monoclonal antibodies specific for mouse kappa light chains and mouse gamma heavy chains have been generated. These rat monoclonal antibodies have been biosynthetically labelled with 35S methionine. The free label was dialyzed from the medium and, without further purification, the medium containing the radioactive monoclonal antibody was used in a radioimmunoassay to screen the sera of the immunized animals and hybridomas for specific mouse antibodies of the IgG class.Entities:
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Year: 1981 PMID: 6821395 DOI: 10.1089/hyb.1.1981.1.5
Source DB: PubMed Journal: Hybridoma ISSN: 0272-457X