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Literature DB >> 6819862

A correlation between protein thermostability and resistance to proteolysis.

R M Daniel, D A Cowan, H W Morgan, M P Curran.

Abstract

The loss of activity due to proteolysis of purified L-asparaginase and beta-galactosidase from different sources correlates with the thermal instability of the enzymes. A similar correlation is found when populations of soluble proteins from micro-organisms grown at different temperatures are compared for proteolytic susceptibility and thermal stability. It is proposed that there is a general correlation between the thermostability of proteins and their resistance to proteolysis.

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Year: 1982 PMID： 6819862 PMCID： PMC1153914 DOI： 10.1042/bj2070641

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors: G R CRAVEN; E STEERS; C B ANFINSEN
Journal: J Biol Chem Date: 1965-06 Impact factor: 5.157

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

Review 3. Mechanisms of thermophily.

Authors: R E Amelunxen; A L Murdock
Journal: CRC Crit Rev Microbiol Date: 1978

Review 4. Intracellular protein degradation in mammalian and bacterial cells.

Authors: A L Goldberg; J F Dice
Journal: Annu Rev Biochem Date: 1974 Impact factor: 23.643

5. Anaerobic-nitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P 450 , other haemoproteins, nitrate and nitrite reductases.

Authors: R M Daniel; C A Appleby
Journal: Biochim Biophys Acta Date: 1972-09-20

6. Genetic and enzymatic experiments relating to the tertiary structure of beta-galactosidase.

Authors: J Langridge
Journal: J Bacteriol Date: 1968-11 Impact factor: 3.490

Review 7. Stability of proteins: small globular proteins.

Authors: P L Privalov
Journal: Adv Protein Chem Date: 1979

8. Molecular basis of thermostability in the lysozyme from bacteriophage T4.

Authors: M G Grütter; R B Hawkes; B W Matthews
Journal: Nature Date: 1979-02-22 Impact factor: 49.962

9. The conformation of thermolysin.

Authors: B W Matthews; L H Weaver; W R Kester
Journal: J Biol Chem Date: 1974-12-25 Impact factor: 5.157

10. Conformational change with temperature and thermostability of glutamine synthetase from Bacillus stearothermophilus.

Authors: A Matsunaga; Y Noso
Journal: Biochim Biophys Acta Date: 1974-09-13

26 in total

1. Probing protein stability and proteolytic resistance by loop scanning: a comprehensive mutational analysis.

Authors: Shoeb Ahmad; Virender Kumar; K Bhanu Ramanand; N Madhusudhana Rao
Journal: Protein Sci Date: 2012-02-06 Impact factor: 6.725

2. Automated selection of stabilizing mutations in designed and natural proteins.

Authors: Benjamin Borgo; James J Havranek
Journal: Proc Natl Acad Sci U S A Date: 2012-01-17 Impact factor: 11.205

3. Proteolysin, a novel highly thermostable and cosolvent-compatible protease from the thermophilic bacterium Coprothermobacter proteolyticus.

Authors: Ana Toplak; Bian Wu; Fabrizia Fusetti; Peter J L M Quaedflieg; Dick B Janssen
Journal: Appl Environ Microbiol Date: 2013-07-12 Impact factor: 4.792

A correlation between protein thermostability and resistance to proteolysis.

1. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Review 3. Mechanisms of thermophily.

Review 4. Intracellular protein degradation in mammalian and bacterial cells.

5. Anaerobic-nitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P 450 , other haemoproteins, nitrate and nitrite reductases.

6. Genetic and enzymatic experiments relating to the tertiary structure of beta-galactosidase.

Review 7. Stability of proteins: small globular proteins.

8. Molecular basis of thermostability in the lysozyme from bacteriophage T4.

9. The conformation of thermolysin.

10. Conformational change with temperature and thermostability of glutamine synthetase from Bacillus stearothermophilus.

1. Probing protein stability and proteolytic resistance by loop scanning: a comprehensive mutational analysis.

2. Automated selection of stabilizing mutations in designed and natural proteins.

3. Proteolysin, a novel highly thermostable and cosolvent-compatible protease from the thermophilic bacterium Coprothermobacter proteolyticus.

4. Isolation of cellulolytic anaerobic extreme thermophiles from new zealand thermal sites.

Review 5. The denaturation and degradation of stable enzymes at high temperatures.

6. Dynamics and unfolding pathways of a hyperthermophilic and a mesophilic rubredoxin.

7. Bulky Dehydroamino Acids Enhance Proteolytic Stability and Folding in β-Hairpin Peptides.

8. A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme.

Review 9. Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases.

10. A comparison of tomato (Lycopersicon esculentum) lectin with its deglycosylated derivative.