Literature DB >> 6819817

A method for the hyaluronic acid synthetase assay in human skin.

H Larjava, J Vaarala, H Saarni, R Penttinen, V K Hopsu-Havu.   

Abstract

A method for the determination of hyaluronic acid synthetase activity of human skin is described. Skin samples were crush-homogenized, and incubated with UDP-14C-glucuronic acid and UDP-N-acetylglucosamine in Tris-HCl-buffer containing MgCl2. After papain digestion of the samples, the 14C-labeled hyaluronic acid was separated in Sephadex G 50 chromatography. The radioactivity incorporated into hyaluronic acid was an expression of the activity of hyaluronic acid synthetase. This activity was found to be increased in skin biopsies obtained from psoriatic lesions and decreased after local treatment with potent corticoids.

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Year:  1982        PMID： 6819817     DOI： 10.1007/bf00409247

Source DB:  PubMed          Journal:  Arch Dermatol Res        ISSN： 0340-3696            Impact factor:   3.017


  13 in total

Review 1.  Defects in the biochemistry of collagen in diseases of connective tissue.

Authors:  J Uitto; J R Lichtenstein
Journal:  J Invest Dermatol       Date:  1976-02       Impact factor: 8.551

Review 2.  Biosynthesis of collagen and its alterations in pathological states.

Authors:  K I Kivirikko; L Risteli
Journal:  Med Biol       Date:  1976-06

3.  A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium.

Authors:  H Saarni; M Tammi
Journal:  Anal Biochem       Date:  1977-07       Impact factor: 3.365

4.  Synthesis of hyaluronic acid by a soluble enzyme system from mammalian tissue.

Authors:  S Schiller
Journal:  Biochem Biophys Res Commun       Date:  1964-03-26       Impact factor: 3.575

5.  Decreased protein degradation in the skin of glucocorticoid-treated newborn rats.

Authors:  K R Cutroneo; D H Clarke
Journal:  Biochem Pharmacol       Date:  1979-11-01       Impact factor: 5.858

6.  Synthesis of hyaluronic acid and collagen in skin fibroblasts cultured from patients with osteogenesis imperfecta.

Authors:  H Turakainen; H Larjava; H Saarni; R Penttinen
Journal:  Biochim Biophys Acta       Date:  1980-04-03

7.  Analysis of aortic glycosaminoglycans from various animal species by CPC-cellulose column procedures.

Authors:  S Thunell; C A Antonopoulos; S Gardell
Journal:  J Atheroscler Res       Date:  1967 May-Jun

8.  The metabolism of mucopolysaccharides in animals. III. Further studies on skin utilizing C14-glucose, C14-acetate, and S35-sodium sulfate.

Authors:  S SCHILLER; M B MATHEWS; J A CIFONELLI; A DORFMAN
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

9.  Cortisol decreases the synthesis of hyaluronic acid by human aortic smooth muscle cells in culture.

Authors:  H Larjava; H Saarni; M Tammi; R Penttinen; T Rönnemaa
Journal:  Atherosclerosis       Date:  1980-02       Impact factor: 5.162

10.  Effect of five anti-inflammatory steroids on collagen and glycoaminoglycan synthessis in vitro.

Authors:  H Saarni; M Jalkanen; V K Hopsu-Havu
Journal:  Br J Dermatol       Date:  1980-08       Impact factor: 9.302
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  1 in total

1.  Quantitative continuous assay for hyaluronan synthase.

Authors:  Joanne C Krupa; David Shaya; Lianli Chi; Robert J Linhardt; Miroslaw Cygler; Stephen G Withers; John S Mort
Journal:  Anal Biochem       Date:  2006-11-27       Impact factor: 3.365
  1 in total

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