Cytoarchitecture, neuronal morphology, and some efferent connections of the interstitial nucleus of Cajal (INC) in the cat.

This study describes the cytoarchitecture and neuronal morphology of the interstitial nucleus of Cajal (INC) in the cat. In addition, the efferent projections of this nucleus to the spinal cord and inferior olive were studied by retrograde labelling with horseradish peroxidase (HRP). The INC was shown to extend rostrocaudally for slightly more than 2 mm. Caudally, the nucleus consists of a small number of loosely aggregated neurons lying lateral to the ventral periaqueductal gray matter at a rostrocaudal level corresponding to the rostral one-fifth of the somatic cell columns of the oculomotor nucleus. Rostrally, the INC increases in size and reaches its maximum development in its rostral half, where it lies ventrolateral to the nucleus of Darkschewitsch (ND). Rostrally the INC is bounded by the dorsoventrally aligned fibres of the fasciculus retroflexus. Two groups of neurons could be distinguished within the INC in both normal and HRP-injected material. One group consists of a relatively small number of large, oval, pyramidal, fusiform, or multipolar neurons with mean dimensions of 40 X 26 micrometers. The second group consists of numerous small to medium-sized neurons with mean dimensions of 20 X 14 micrometers. Large neurons and some cells of the second group contain substantial amounts of Nissl substance throughout their perikarya. Some medium-sized to small neurons exhibit indentations in their nuclei, and glial cells are often apposed to their cell membranes. Golgi-Kopsch preparations taken from kitten showed that INC neurons possess sparsely branched, radiating dendritic trees with few spinous processes. The majority of INC neurons retrogradely labelled with HRP exhibited similar dendritic patterns. Injections of HRP into lesions at cervical, thoracic, or lumbar levels of the spinal cord resulted in retrograde labelling of neurons of all sizes and shapes throughout the entire length of the INC. However, the greatest number of HRP-labelled cells in INC were observed subsequent to injections of the enzyme into cervical levels of the cord. Following injections of HRP into the inferior olive only small to medium-sized neurons were labelled in the nucleus, the majority of which are located in rostral levels of the INC. A substantial olivary projection was observed to originate in the nucleus of Darkschewitsch (ND) and the nucleus parafascicularis (NPF). The sizes of the projections from these two nuclei to the inferior olive appeared to be much larger than that from the INC. Smaller numbers of neurons were also observed in the rostral parvocellular red nucleus (RN) and mesencephalic reticular formation (MRF).