Stimulants of prostaglandin biosynthesis in human fetal membranes, uterine decidua vera and placenta.

Modulation of prostaglandin (PG) biosynthesis by cytosolic fractions derived from homogenates of human amnion, chorion laeve, decidua vera and placenta was examined. PGF2 alpha and 6-oxo-PGF1 alpha synthesis by bovine seminal vesicle (BSV) PG synthase was stimulated by the cytosolic fractions of each tissue in a dose-dependent manner. The cytosols from decidua vera and placenta were the most effective in stimulating synthesis and also stimulated PGE2 biosynthesis. Reduced glutathione (GSH) acted to increase the biosynthesis of PGE2 at the expense of other PGs both in the presence and absence of various cytosols. These data are indicative that the mode of action of cytosolic fractions on the stimulation of PG biosynthesis is unlike that of GSH. Indomethacin and aspirin, inhibitors of fatty acid cyclo-oxygenase activity, strongly inhibited the cytosol-induced stimulation of BSV PG synthase. The cytosolic factors that stimulated PG biosynthesis exhibited differential behavior towards boiling and dialysis. The stimulatory effect of all cytosolic fractions was sensitive to boiling except in the the chorion laeve effects toward 6-oxo-PGF1 alpha production. In dialysis studies we found that the cytosolic components that stimulated the production of PGF2 alpha were not removed by dialysis except in the case of cytosol of placenta whereas the stimulatory effects of various cytosols toward the biosynthesis of PGE2 and 6-oxo-PGF1 alpha were removed by dialysis. These results are indicative of the presence of endogenous factors in human intrauterine tissues that preferentially stimulate the biosynthesis of PGF2 alpha and 6-oxo-PGF1 alpha and are further suggestive that PG biosynthesis in intrauterine tissues is, at least in part, regulated by cytosolic factors.