| Literature DB >> 6817079 |
S J Silverman, M Rose, D Botstein, G R Fink.
Abstract
The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.Entities:
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Year: 1982 PMID: 6817079 PMCID: PMC369920 DOI: 10.1128/mcb.2.10.1212-1219.1982
Source DB: PubMed Journal: Mol Cell Biol ISSN: 0270-7306 Impact factor: 4.272