| Literature DB >> 6816855 |
A M Konijn, R Levy, G Link, C Hershko.
Abstract
A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-beta-D-galactopyranoside as the substrate for beta-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-beta-galactosidase conjugate. The procedure is performed in microELISA plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 microgram/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.Entities:
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Year: 1982 PMID： 6816855 DOI： 10.1016/0022-1759(82)90314-3
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303