Literature DB >> 6816632

Cytoplasmic free Ca2+ in human platelets: Ca2+ thresholds and Ca-independent activation for shape-change and secretion.

T J Rink, S W Smith, R Y Tsien.   

Abstract

Cytoplasmic free [Ca2+], [Ca2+]i, was measured in human platelets using the intracellularly-trapped, fluorescent indicator quin2. Basal [Ca2+]i with the Ca2+ -ionophore ionomycin revealed apparent thresholds for shape-change, 5-HT release and aggregation of approx. 0.5 microM, 0.8 microM and 2 microM. Thrombin raised [Ca2+]i to 3 microM fast enough for the Ca2+ to have triggered the cell activation. However, thrombin released more 5-HT than ionomycin could, and in Ca2+ -free medium thrombin evoked shape-change and secretion even when [Ca2+]i remained near basal levels throughout, suggesting the existence of alternative triggers for shape-change and secretory exocytosis.

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Year:  1982        PMID： 6816632     DOI： 10.1016/0014-5793(82)81234-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  78 in total

1.  Effects of temperature on calcium-sensitive fluorescent probes.

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Authors:  J Anagli; J Hagmann; E Shaw
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857

3.  Low density lipoprotein causes general cellular activation with increased phosphatidylinositol turnover and lipoprotein catabolism.

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Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

4.  Effects of the calmodulin antagonists fendiline and calmidazolium on aggregation, secretion of ATP, and internal calcium in washed human platelets.

Authors:  A Lückhoff; M Bohnert; R Busse
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1991-01       Impact factor: 3.000

5.  Effects of captopril on platelet cytosolic free Ca2+ concentrations and on suppression of cell proliferation in culture.

Authors:  D W Wang; H Y Zhao; S L Yang; Z D Deng
Journal:  J Tongji Med Univ       Date:  1993

6.  Stimulus-response coupling in human platelets. Changes evoked by platelet-activating factor in cytoplasmic free calcium monitored with the fluorescent calcium indicator quin2.

Authors:  T J Hallam; A Sanchez; T J Rink
Journal:  Biochem J       Date:  1984-03-15       Impact factor: 3.857

7.  Ca2+ as messenger of 5HT2-receptor stimulation in human blood platelets.

Authors:  H Affolter; P Erne; E Bürgisser; A Pletscher
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1984-04       Impact factor: 3.000

8.  Ca(2+)-dependent aggregation of rabbit platelets induced by maitotoxin, a potent marine toxin, isolated from a dinoflagellate.

Authors:  A Watanabe; Y Ishida; H Honda; M Kobayashi; Y Ohizumi
Journal:  Br J Pharmacol       Date:  1993-05       Impact factor: 8.739

9.  Monitoring of cytosolic free Ca2+ in C5a-stimulated neutrophils: loss of receptor-modulated Ca2+ stores and Ca2+ uptake in granule-free cytoplasts.

Authors:  R Gennaro; T Pozzan; D Romeo
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

10.  myo-Inositol 1,4,5-trisphosphate stimulates protein phosphorylation in saponin-permeabilized human platelets.

Authors:  E G Lapetina; S P Watson; P Cuatrecasas
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205
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