Nature of the antigenic complex recognized by T lymphocytes. IX. Direct immunochemical demonstration of nominal antigen on the macrophage cell surface.

As an initial approach to macrophage (M phi) antigen handling, guinea pig M phi pulsed with the radiolabeled terpolymer L-glutamic acid, L-lysine, L-tyrosine (GLT) were modified with the cell surface-specific probe 2,4,6-trinitrobenzenesulfonic acid either immediately after pulsing (fresh) or 24 h after pulsing (aged); nonidet P-40 extracts were prepared from these cells and analysis of these extracts with anti-trinitrophenyl antisera was performed in a quantitative radioimmunoassay. Such experiments indicated that fresh GLT-pulsed M phi contained a pool of intracellular GLT as well as a distinct pool of cell surface GLT. In contrast, aged GLT-pulsed M phi lacked intracellular GLT but expressed GLT on the cell surface. In addition, a pool of cell surface GLT was also detected on Ia-M phi and on M phi from guinea pigs which were nonresponders to GLT. Thus, this study provides a direct demonstration of the presence of exogenous antigen on the M phi cell surface. However, the cell surface expression of GLT does not appear to be an exclusive property expressed by functional Ia+ antigen-presenting M phi.