| Literature DB >> 6815633 |
Abstract
An improved method for spore membranes isolation was developed based on sucrose density gradient centrifugation in a vertical rotor. The advantage of this over previous methods was the complete removal of RNA and a 40% reduction in protein content, while retaining the high specific activities for membrane bound dehydrogenases and for amino acid uptake.Entities:
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Year: 1982 PMID： 6815633 DOI： 10.1080/00327488208065567
Source DB: PubMed Journal: Prep Biochem ISSN： 0032-7484