Literature DB >> 6815206

Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster.

R J Hill, M R Mott, E J Burnett, S M Abmayr, K Lowenhaupt, S C Elgin.   

Abstract

The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded native Drosophila salivary chromosomes. These chromosomes were then swollen and spread by a modified Miller procedure, stained or shadowed, and examined in the electron microscope. Individual nucleoprotein fibers were resolved with regularly repeated nucleosomes of approximately 10 nm diameter. Micrococcal nuclease digestion of isolated salivary nuclei gave a family of DNA fragments characteristic of nucleosomes for total chromatin, 5S gene, and simple satellite (rho = 1.688 g/cm3) sequences.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6815206      PMCID： PMC2112364          DOI： 10.1083/jcb.95.1.262

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  11 in total

1.  Electron microscopy of whole mount metaphase chromosomes.

Authors:  J B Rattner; A Branch; B A Hamkalo
Journal:  Chromosoma       Date:  1975-11-11       Impact factor: 4.316

2.  Chromatin structure: deduced from a minichromosome.

Authors:  J D Griffith
Journal:  Science       Date:  1975-03-28       Impact factor: 47.728

3.  Sequence and sequence variation within the 1.688 g/cm3 satellite DNA of Drosophila melanogaster.

Authors:  T Hsieh; D Brutlag
Journal:  J Mol Biol       Date:  1979-12-05       Impact factor: 5.469

4.  "Native" salivary chromosomes of Drosophila melanogaster.

Authors:  R J Hill; F Watt
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

5.  The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.

Authors:  C Wu; P M Bingham; K J Livak; R Holmgren; S C Elgin
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

6.  Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

Authors:  D R Hewish; L A Burgoyne
Journal:  Biochem Biophys Res Commun       Date:  1973-05-15       Impact factor: 3.575

7.  Visualization of nucleolar genes.

Authors:  O L Miller; B R Beatty
Journal:  Science       Date:  1969-05-23       Impact factor: 47.728

8.  Organization of chromatin during spermiogenesis: beaded fibers, partly beaded fibers, and loss of nucleosomal structure.

Authors:  R McMaster-Kaye; J S Kaye
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

9.  The 5S genes of Drosophila melanogaster.

Authors:  S Artavanis-Tsakonas; P Schedl; C Tschudi; V Pirrotta; R Steward; W J Gehring
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

10.  Visualization of nucleosomes in thin sections by stereo electron microscopy.

Authors:  A L Olins; D E Olins; H Zentgraf; W W Franke
Journal:  J Cell Biol       Date:  1980-12       Impact factor: 10.539
View more
  7 in total

1.  The ultrastructural morphology of native salivary gland chromosomes of Drosophila melanogaster: the band-interband question.

Authors:  M R Mott; R J Hill
Journal:  Chromosoma       Date:  1986       Impact factor: 4.316

2.  Isolation and distribution of a Drosophila protein preferentially associated with active regions of the genome.

Authors:  B Fleischmann; R Filipski; G Fleischmann
Journal:  Chromosoma       Date:  1989-03       Impact factor: 4.316

3.  Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome.

Authors:  G Fleischmann; R Filipski; S C Elgin
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

4.  Ultrastructural analysis of polytene chromatin of Drosophila melanogaster reveals clusters of tightly linked co-expressed genes.

Authors:  E J Hager; O L Miller
Journal:  Chromosoma       Date:  1991-03       Impact factor: 4.316

5.  Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.

Authors:  G Fleischmann; G Pflugfelder; E K Steiner; K Javaherian; G C Howard; J C Wang; S C Elgin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

6.  Three-dimensional organization of Drosophila melanogaster interphase nuclei. II. Chromosome spatial organization and gene regulation.

Authors:  M Hochstrasser; J W Sedat
Journal:  J Cell Biol       Date:  1987-06       Impact factor: 10.539

7.  In memoriam: Susan Abmayr (1956-2019) - "What do we do? Whatever it takes!"

Authors:  Erika R Geisbrecht; Mary K Baylies
Journal:  Skelet Muscle       Date:  2019-11-27       Impact factor: 4.912
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.