Literature DB >> 6815171

Purification and characterization of phosphatidylinositol-specific phospholipase C from bovine platelets.

H Hakata, J Kambayashi, G Kosaki.   

Abstract

Phosphatidylinositol-specific phospholipase C was purified to homogeneity from soluble fraction of bovine platelets by ammonium sulfate fractionation, hydrophobic chromatography, DEAE ion exchange chromatography and gel filtration. The purified enzyme has a narrow pH optimum ranging from 6.5 to 7.5 and the molecular weight of the enzyme was estimated to be 143,000 by sodium dodecyl sulfate slab gel electrophoresis. The purified enzyme requires Ca2+ strictly for activity, which was markedly enhanced in the presence of arachidonate. No enhancement of the activity was observed in the presence of purified calmodulin. The activity was markedly inhibited in the presence of quinacrine but no inhibition by indomethacin was observed.

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Year:  1982        PMID： 6815171     DOI： 10.1093/oxfordjournals.jbchem.a134008

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  11 in total

1.  Characterization of partially purified phospholipase C from human platelet membranes.

Authors:  Y Banno; Y Nozawa
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

2.  Characterization of phosphoinositide-specific phospholipase C from human platelets.

Authors:  V Manne; H F Kung
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

3.  Characterization of multiple forms of phosphoinositide-specific phospholipase C purified from human platelets.

Authors:  M G Low; R C Carroll; A C Cox
Journal:  Biochem J       Date:  1986-07-01       Impact factor: 3.857

4.  Multiple forms of phosphoinositide-specific phospholipase C of different relative molecular masses in animal tissues. Evidence for modification of the platelet enzyme by Ca2+-dependent proteinase.

Authors:  M G Low; R C Carroll; W B Weglicki
Journal:  Biochem J       Date:  1984-08-01       Impact factor: 3.857

5.  Ca2+-induced changes in the secondary structure of a 60 kDa phosphoinositide-specific phospholipase C from bovine brain cytosol.

Authors:  C Herrero; M E Cornet; C Lopez; P G Barreno; A M Municio; J Moscat
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

6.  Regulation of sn-1,2-diacylglycerol second-messenger formation in thrombin-stimulated human platelets. Potentiation by protein kinase C inhibitors.

Authors:  W R Bishop; J August; J M Petrin; J K Pai
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

7.  Phospholipase C associated with particulate fractions of bovine brain.

Authors:  K Y Lee; S H Ryu; P G Suh; W C Choi; S G Rhee
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

8.  Purification of two distinct types of phosphoinositide-specific phospholipase C from rat liver. Enzymological and structural studies.

Authors:  O Nakanishi; Y Homma; H Kawasaki; Y Emori; K Suzuki; T Takenawa
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

9.  Resolution of the phosphoinositide-specific phospholipase C isolated from porcine lymphocytes into multiple species. Partial purification of two isoenzymes.

Authors:  H R Carter; A D Smith
Journal:  Biochem J       Date:  1987-06-15       Impact factor: 3.857

10.  Resolution of myocardial phospholipase C into several forms with distinct properties.

Authors:  M G Low; W B Weglicki
Journal:  Biochem J       Date:  1983-11-01       Impact factor: 3.857
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