Literature DB >> 6814954

Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay.

U Aehringhaus, R H Wölbling, W König, C Patrono, B M Peskar, B A Peskar.   

Abstract

Rabbits were immunized with a conjugate of leukotriene (LT) C4 and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [3H]LTC4 to the antibodies obtained is inhibited by 50% with 1.5 ng LTC4. The relative cross-reaction of LTD4 is 16% and of LTC4-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC4 is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.

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Year:  1982        PMID： 6814954     DOI： 10.1016/0014-5793(82)80715-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  38 in total

1.  Modulation of the contractile activity of the guinea-pig lung parenchymal strip by exogenous 5,8,11,14,17-eicosapentaenoic acid.

Authors:  T Simmet; J Aissa; D Sutter; H Juan; B A Peskar
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1987-06       Impact factor: 3.000

2.  Release of cytoprotective PGE2 from cultured macrophages induced by antacids and sucralfate.

Authors:  I Szelenyi; R Lanz
Journal:  Agents Actions       Date:  1986-06

3.  Effect of muscarinic receptor stimulation on release of cysteinyl-leukotrienes and thromboxane B2 from anaphylactic guinea-pig hearts.

Authors:  G Wittmann; P Weinerowski; T Simmet; B A Peskar
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1988-11       Impact factor: 3.000

4.  Interleukin-1 enhances pain reflexes. Mediation through increased prostaglandin E2 levels.

Authors:  A Schweizer; U Feige; A Fontana; K Müller; C A Dinarello
Journal:  Agents Actions       Date:  1988-12

5.  Effects of exogenous prostaglandins on the release of leukotriene C4-like immunoreactivity and on coronary flow in indomethacin-treated anaphylactic guinea-pig hearts.

Authors:  U Aehringhaus; A Dembinska-Kiéc; B A Peskar
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1984-07       Impact factor: 3.000

6.  Effect of thiol-activated toxins (streptolysin O, alveolysin, and theta toxin) on the generation of leukotrienes and leukotriene-inducing and -metabolizing enzymes from human polymorphonuclear granulocytes.

Authors:  K D Bremm; W König; P Pfeiffer; I Rauschen; K Theobald; M Thelestam; J E Alouf
Journal:  Infect Immun       Date:  1985-12       Impact factor: 3.441

7.  Lung responsiveness to antigen in sensitised mice of different strains.

Authors:  C Zuany-Amorim; M Pretolani; D Leduc; R Cordeiro; B B Vargaftig
Journal:  Agents Actions       Date:  1993

8.  Pharmacological control of leukotriene and prostaglandin production from mouse peritoneal macrophages.

Authors:  K Brune; U Aehringhaus; B A Peskar
Journal:  Agents Actions       Date:  1984-06

9.  Preferential generation of leukotriene C4 by human eosinophils.

Authors:  R J Shaw; O Cromwell; A B Kay
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

10.  Leukotriene A4 modulates generation of leukotriene B4 and sulphidopeptide leukotrienes by human neutrophils.

Authors:  R A Hilger; W König
Journal:  Immunology       Date:  1992-11       Impact factor: 7.397
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