Literature DB >> 6813856

In vitro replication of bacteriophage phi 29 DNA.

K Watabe, M F Shih, A Sugino, J Ito.   

Abstract

We have been studying the mechanisms of linear DNA replication by using Bacillus bacteriophage phi 29 as a model system. To isolate and characterize the proteins required for phi 29 DNA replication, we have developed a cell-free replication system. A cell-free extract prepared from phi 29-infected Bacillus subtilis catalyzes the semiconservative replication of phi 29 DNA, but only if exogenous phi 29 DNA-protein complex is used as the template. This template consists of linear duplex DNA with a 30,000-dalton terminal protein attached covalently to both 5' ends. Replication starts nonsimultaneously at or near both ends of the template. The extract also catalyzes the specific binding between dATP and the phi 29 terminal protein. Thus, the in vitro system closely mimics the in vivo replication of phi 29 DNA. This system should allow characterization of the phi 29 DNA replication machinery.

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Year:  1982        PMID： 6813856      PMCID： PMC346872          DOI： 10.1073/pnas.79.17.5245

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA.

Authors:  C O Yehle
Journal:  J Virol       Date:  1978-09       Impact factor: 5.103

2.  The infectivity of adenovirus 5 DNA-protein complex.

Authors:  P A Sharp; C Moore; J L Haverty
Journal:  Virology       Date:  1976-12       Impact factor: 3.616

3.  Thermolabile transfecting DNA from temperature-sensitive mutant of phage phi29.

Authors:  S Yanofsky; F Kawamura; J Ito
Journal:  Nature       Date:  1976 Jan 1-8       Impact factor: 49.962

4.  Identification of a protein linked to the ends of adenovirus DNA.

Authors:  D M Rekosh; W C Russell; A J Bellet; A J Robinson
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

5.  Studies of Bacillus subtilis phage M2: a physical map of the M2 genome.

Authors:  F Kawamura; J Ito
Journal:  Virology       Date:  1977-12       Impact factor: 3.616

6.  Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29.

Authors:  M Salas; R P Mellado; E Viñuela
Journal:  J Mol Biol       Date:  1978-02-25       Impact factor: 5.469

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Analysis of gene function of bacteriophage phi 29 of Bacillus subtilis: identification of cistrons essential for viral assembly.

Authors:  E W Hagen; B E Reilly; M E Tosi; D L Anderson
Journal:  J Virol       Date:  1976-08       Impact factor: 5.103

9.  Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.

Authors:  R P Mellado; F Moreno; E Viñuela; M Salas; B E Reilly; D L Anderson
Journal:  J Virol       Date:  1976-08       Impact factor: 5.103

10.  Bacillus subtilis phage phi29. Characterization of gene products and functions.

Authors:  J L Carrascosa; A Camacho; F Moreno; F Jiménez; R P Mellado; E Viñuela; M Salas
Journal:  Eur J Biochem       Date:  1976-07-01
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  15 in total

1.  Formation of a covalent complex between the terminal protein of pneumococcal bacteriophage Cp-1 and 5'-dAMP.

Authors:  P García; J M Hermoso; J A García; E García; R López; M Salas
Journal:  J Virol       Date:  1986-04       Impact factor: 5.103

2.  Overproduction and purification of protein P6 of Bacillus subtilis phage phi 29: role in the initiation of DNA replication.

Authors:  R Pastrana; J M Lázaro; L Blanco; J A García; E Méndez; M Salas
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

3.  Identification and characterization of a protein covalently bound to DNA of minute virus of mice.

Authors:  M Chow; J W Bodnar; M Polvino-Bodnar; D C Ward
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

4.  Purification in a functional form of the terminal protein of Bacillus subtilis phage phi 29.

Authors:  I Prieto; J M Lázaro; J A García; J M Hermoso; M Salas
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  A novel DNA polymerase induced by Bacillus subtilis phage phi 29.

Authors:  K Watabe; J Ito
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

6.  Efficient isolation of the linear DNA killer plasmid of Kluyveromyces lactis: evidence for location and expression in the cytoplasm and characterization of their terminally bound proteins.

Authors:  J C Stam; J Kwakman; M Meijer; A R Stuitje
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971

7.  Replication of bacteriophage phi 29 DNA in vitro: the roles of terminal protein and DNA polymerase.

Authors:  K Watabe; M Leusch; J Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

8.  Primary structure of the DNA terminal protein of bacteriophage PRD1.

Authors:  J C Hsieh; G H Jung; M C Leavitt; J Ito
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

9.  Protein-primed initiation of phage phi 29 DNA replication.

Authors:  K Watabe; M Shin; J Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

10.  Replication of phage phi 29 DNA with purified terminal protein and DNA polymerase: synthesis of full-length phi 29 DNA.

Authors:  L Blanco; M Salas
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205
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