Literature DB >> 6812651

Cloning of a nitrogen fixation (nif) gene cluster of Azospirillum brasilense.

B Quiviger, C Franche, G Lutfalla, D Rice, R Haselkorn, C Elmerich.   

Abstract

Homology was detected between the structural genes for the nitrogenase complex of K. pneumoniae (nifHDK genes) and the total DNA of several Azospirillum strains. Bacteriophage lambda gt 7-ara6 was used to construct a gene bank of A. brasilense strain 7000 DNA and a recombinant phage carrying a 6.7 kb Eco RI fragment, termed AbRI, was selected by hybridization with the K. pneumoniae nif probe. Using heteroduplex analysis the extent of the homology of the AbRI fragment and the K. pneumoniae nif genes was found to be approximately 5 kb. Proteins encoded by the AbRI fragment were examined after infection of E. coli minicells.

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Year:  1982        PMID: 6812651     DOI: 10.1016/s0300-9084(82)80165-x

Source DB:  PubMed          Journal:  Biochimie        ISSN: 0300-9084            Impact factor:   4.079


  22 in total

1.  Analysis of the HO-cleaved MAT DNA intermediate generated during the mating type switch in the yeast Saccharomyces cerevisiae.

Authors:  D Raveh; S H Hughes; B K Shafer; J N Strathern
Journal:  Mol Gen Genet       Date:  1989-12

2.  A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA.

Authors:  E Leibovitz; P Béguin
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

3.  The nifEN genes participating in FeMo cofactor biosynthesis and genes encoding dinitrogenase are part of the same operon in Bradyrhizobium species.

Authors:  O M Aguilar; J Taormino; B Thöny; T Ramseier; H Hennecke; A A Szalay
Journal:  Mol Gen Genet       Date:  1990-12

4.  Isolation and Characterization of Frankia sp. Strain FaC1 Genes Involved in Nitrogen Fixation.

Authors:  J M Ligon; J P Nakas
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

5.  Transposon Mutagenesis of Azospirillum brasilense and Azospirillum lipoferum: Physical Analysis of Tn5 and Tn5-Mob Insertion Mutants.

Authors:  M Vanstockem; K Michiels; J Vanderleyden; A P Van Gool
Journal:  Appl Environ Microbiol       Date:  1987-02       Impact factor: 4.792

6.  Role of scaffolding protein CipC of Clostridium cellulolyticum in cellulose degradation.

Authors:  S Pagès; L Gal; A Bélaïch; C Gaudin; C Tardif; J P Bélaïch
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

7.  Regulation of transcription and promoter mapping of the structural genes for nitrogenase (nifHDK) of Azospirillum brasilense Sp7.

Authors:  M de Zamaroczy; F Delorme; C Elmerich
Journal:  Mol Gen Genet       Date:  1989-12

8.  Identification of a regulatory nifA type gene and physical mapping of cloned new nif regions of Azospirillum brasilense.

Authors:  M Singh; A K Tripathi; W Klingmüller
Journal:  Mol Gen Genet       Date:  1989-10

9.  Purification and characterization of glutamate synthase from Azospirillum brasilense.

Authors:  S Ratti; B Curti; G Zanetti; E Galli
Journal:  J Bacteriol       Date:  1985-08       Impact factor: 3.490

10.  DNA hybridization analysis of the nif region of two methylotrophs and molecular cloning of nif-specific DNA.

Authors:  A E Toukdarian; M E Lidstrom
Journal:  J Bacteriol       Date:  1984-03       Impact factor: 3.490

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