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Literature DB >> 6810872

Purification and genetic control of NAD-dependent glutamate dehydrogenase from Drosophila melanogaster.

Abstract

Glutamate dehydrogenase has been purified to near-homogeneity from mature larvae of Drosophila melanogaster. The enzyme has a molecular weight of 347,000 measured by sucrose gradient sedimentation and 343,000 measured by variable-porosity acrylamide gel electrophoresis. Electrophoresis under denaturing conditions showed that the enzyme consists of six subunits of molecular weight 57,000. The structural gene for GDH has been mapped at 81.7 +/- 0.8 on the third chromosome by means of an electrophoretic variant.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6810872 DOI： 10.1007/bf00484696

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

14 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

3. [Glutamate dehydrogenase. Attachment of NAD and NADP coenzymes and other nucleotides derived from adenosine-5'-phosphate].

Authors: D Pantaloni; P Dessen
Journal: Eur J Biochem Date: 1969-12

4. Purification and kinetic characteristics of dogfish liver glutamate dehydrogenase.

Authors: L Corman; L M Prescott; N O Kaplan
Journal: J Biol Chem Date: 1967-04-10 Impact factor: 5.157

5. Glutamate dehydrogenase of Drosophila larvae.

Authors: P A Bond; J H Sang
Journal: J Insect Physiol Date: 1968-03 Impact factor: 2.354

6. Sequence of bovine liver glutamate dehydrogenase. 8. Peptides produced by specific chemical cleavages; the complete sequence of the protein.

Authors: K Moon; E L Smith
Journal: J Biol Chem Date: 1973-05-10 Impact factor: 5.157

Purification and genetic control of NAD-dependent glutamate dehydrogenase from Drosophila melanogaster.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

3. [Glutamate dehydrogenase. Attachment of NAD and NADP coenzymes and other nucleotides derived from adenosine-5'-phosphate].

4. Purification and kinetic characteristics of dogfish liver glutamate dehydrogenase.

5. Glutamate dehydrogenase of Drosophila larvae.

6. Sequence of bovine liver glutamate dehydrogenase. 8. Peptides produced by specific chemical cleavages; the complete sequence of the protein.

7. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

8. The purification and physical properties of glutamate dehydrogenase from rat liver.

9. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

10. Protein and enzyme variation in Drosophila.

1. The gene coding for glutamate dehydrogenase in Drosophila melanogaster.