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Literature DB >> 680640

[Improved preparation of des-alanylB30-insulin (author's transl)].

Abstract

Des-AlaB30-insulin was prepared by incubation of insulin with carboxypeptidase A. The C-terminal alanineB30 of the B-chain was quantitatively liberated without liberating asparagineA21 of the A-chain in ammonium hydrogencarbonate buffer.

Entities: Chemical Gene

Mesh：

Substances：
Insulin
Carboxypeptidases

Year: 1978 PMID： 680640

Source DB: PubMed Journal: Hoppe Seylers Z Physiol Chem ISSN： 0018-4888

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Cited

8 in total

1. Influence of temperature on the enzymic semisynthesis of human insulin by coupling and transpeptidation methods.

Authors: K Morihara; Y Ueno; K Sakina
Journal: Biochem J Date: 1986-12-15 Impact factor: 3.857

2. A mass-spectrometric investigation of the mechanism of the semisynthetic transformation of pig insulin into an ester of insulin of human sequence.

Authors: K Rose; J Gladstone; R E Offord
Journal: Biochem J Date: 1984-05-15 Impact factor: 3.857

3. Xid-defective male (CBA/N X C57BL/6)F1 accessory cells present bovine insulin to long-term cultured F1-restricted T-cells.

Authors: A B Reske-Kunz; I Iskandar; H G Gattner; D Brandenburg; E Rüde
Journal: Immunogenetics Date: 1982 Impact factor: 2.846

4. Albumin binding of insulins acylated with fatty acids: characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effect in vivo.

Authors: P Kurtzhals; S Havelund; I Jonassen; B Kiehr; U D Larsen; U Ribel; J Markussen
Journal: Biochem J Date: 1995-12-15 Impact factor: 3.857

[Improved preparation of des-alanylB30-insulin (author's transl)].

1. Influence of temperature on the enzymic semisynthesis of human insulin by coupling and transpeptidation methods.

2. A mass-spectrometric investigation of the mechanism of the semisynthetic transformation of pig insulin into an ester of insulin of human sequence.

3. Xid-defective male (CBA/N X C57BL/6)F1 accessory cells present bovine insulin to long-term cultured F1-restricted T-cells.

4. Albumin binding of insulins acylated with fatty acids: characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effect in vivo.

5. [Protein semisynthesis with the help of mixed anhydrides and enzymes: chemistry and synthesis of insulins].

6. Advantages and pitfalls of radioimmune and enzyme linked immunosorbent assays of insulin antibodies.

7. Rapid preparation of human insulin and insulin analogues in high yield by enzyme-assisted semi-synthesis.

Review 8. New approaches to therapy and diagnosis of diabetes.