The influence of protein deprivation during development on amino acid incorporation into cerebral proteins.

The incorporation of 3H-valine into protein of cerebrum, liver, and muscle was studied in rats fed a low protein diet. Animals, 15-50 days old, were used to cover the period during and after the most rapid phase of cerebral protein synthesis. The precursor was administered in doses resulting in brain concentrations of free valine in the 0.1-0.3 mM range. Two series of experiments were run and the injected dose varied for comparison of protein synthesis at different concentrations of precursor valine. By concomitant amino-acid analysis it was shown that the concentration of other amino-acids in the brain was not changed by the injected valine. The changes in the amino-acid pool with age in control rats were in agreement with previous findings. Only small alterations were seen in the amino acid pool as an effect of protein deprivation, the results being consistent with those of some previous reports. Uptake of valine into the brain appeared to be decreased only in 15-17-day-old protein-deprived rats. Valine incorporation into cerebral protein was decreased in the youngest age group but no effect was seen in older animals. Liver protein incorporation was slightly decreased by low protein diet but a marked reduction was observed for muscle protein. The changes in brain protein synthesis during normal development were in agreement with previous studies and differed qualitatively from those in liver and muscle.