Literature DB >> 6803761

Affinity chromatography and immunosorption with acetylcholine receptor attached to nylon tubes.

B H Yang, P V Sundaram, A Maelicke.   

Abstract

Nylon-linked proteins were used for affinity trapping and chromatography. As representative examples purified acetylcholine receptor, alpha-cobratoxin and bovine serum albumin were coupled to the activated matrix to serve as biospecific ligands. In particular, acetylcholine receptor was coupled without significant loss of biochemical properties. The resulting affinity tubes bind receptor-specific ligands including immunoglobulins and thus can be used for affinity-chromatographic purposes and immunoassays.

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Year:  1981        PMID: 6803761      PMCID: PMC1163374          DOI: 10.1042/bj1990317

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  13 in total

1.  Preparation and properties of urease chemically attached to nylon tube.

Authors:  P V. Sundaram; W E. Hornby
Journal:  FEBS Lett       Date:  1970-10       Impact factor: 4.124

2.  Metabolism of acetylcholine receptor in chick embryo muscle cells: effects of RSV and PMA.

Authors:  R Miskin; T G Easton; A Maelicke; E Reich
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

3.  Acetylcholine receptor degradation in adult rat diaphragms in organ culture and the effect of anti-acetylcholine receptor antibodies.

Authors:  J P Merlie; S Heinemann; J M Lindstrom
Journal:  J Biol Chem       Date:  1979-07-25       Impact factor: 5.157

4.  Cytophilic antibodies in experimental autoimmune myasthenia gravis.

Authors:  R D Martinez; R Tarrab-Hazdai; A Aharonov; S Fuchs
Journal:  J Immunol       Date:  1977-01       Impact factor: 5.422

5.  Fluorescein isothiocyanate-labeled alpha-cobratoxin. Biochemical characterization and interaction with acetylcholine receptor from Electrophorus electricus.

Authors:  S Kang; A Maelicke
Journal:  J Biol Chem       Date:  1980-08-10       Impact factor: 5.157

6.  Three-dimensional structure of the "long" neurotoxin from cobra venom.

Authors:  M D Walkinshaw; W Saenger; A Maelicke
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

7.  Monoclonal antibodies directed against the neurotransmitter binding site of nicotinic acetylcholine receptor.

Authors:  R W James; A C Kato; M J Rey; B W Fulpius
Journal:  FEBS Lett       Date:  1980-10-20       Impact factor: 4.124

8.  Translational diffusion coefficient and molecular weight of the acetylcholine receptor from Torpedo marmorata.

Authors:  W Doster; B Hess; D Watters; A Maelicke
Journal:  FEBS Lett       Date:  1980-05-05       Impact factor: 4.124

9.  Immunochemical similarities between subunits of acetylcholine receptors from Torpedo, Electrophorus, and mammalian muscle.

Authors:  J Lindstrom; B Walter; B Einarson
Journal:  Biochemistry       Date:  1979-10-16       Impact factor: 3.162

10.  Monoclonal antibodies used to probe acetylcholine receptor structure: localization of the main immunogenic region and detection of similarities between subunits.

Authors:  S J Tzartos; J M Lindstrom
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

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  1 in total

1.  Suitable hollow fibre immunobioreactors for specific ex vivo removal of antibodies and antigens from plasma.

Authors:  C Larue; V Gueraud; C Rivat
Journal:  Clin Exp Immunol       Date:  1985-10       Impact factor: 4.330

  1 in total

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