Literature DB >> 6802872

Rapid micromethod for preparation of enzyme-antibody conjugates.

A J Parkinson, E N Scott, H G Muchmore.   

Abstract

A rapid method is described for labeling antibody with alkaline phosphatase by one-step glutaraldehyde linkage. The method involves the centrifugation of a small volume of an enzyme and antibody mixture through a minicolumn packed with hydrated Sephadex. This procedure rapidly removes ammonium sulfate and glutaraldehyde from the enzyme-antibody mixture and results in the efficient recovery of conjugated antibody without significant dilution.

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Year:  1982        PMID： 6802872      PMCID： PMC272177          DOI： 10.1128/jcm.15.4.737-739.1982

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  A rapid method for the purification of antibody--enzyme conjugates.

Authors:  M Pagé; M Audette; M Caron
Journal:  Can J Biochem       Date:  1979-03

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  A rapid method for desalting small volumes of solution.

Authors:  M W Neal; J R Florini
Journal:  Anal Biochem       Date:  1973-09       Impact factor: 3.365

4.  A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.

Authors:  G N Bowers; R B McComb
Journal:  Clin Chem       Date:  1966-02       Impact factor: 8.327

5.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors:  S Avrameas
Journal:  Immunochemistry       Date:  1969-01

6.  A simple centrifuge column for desalting protein solutions.

Authors:  R I Christopherson; M E Jones; L R Finch
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

Review 7.  Protein-protein coupling reactions and the applications of protein conjugates.

Authors:  J H Kennedy; L J Kricka; P Wilding
Journal:  Clin Chim Acta       Date:  1976-07-01       Impact factor: 3.786
  7 in total
  1 in total

1.  Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia.

Authors:  K M Rudolph; A J Parkinson; C M Black; L W Mayer
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948
  1 in total

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