Literature DB >> 6801066

Reversed-phase ion-pair liquid chromatographic procedure for the simultaneous analysis of S-adenosylmethionine, its metabolites and the natural polyamines.

J Wagner, C Danzin, P Mamont.   

Abstract

A method using reversed-phase ion-pair liquid chromatography with dual detection was developed for the simultaneous determination of the S-adenosylmethionine (SAM) analogues and the natural polyamines. The separation is obtained with a gradient elution and by adjusting the concentration of octanesulfonic acid used as ion-pairing agent, the ionic strength of the eluent, the pH and the acetonitrile content of the eluents. The SAM analogues are analyzed by UV detection at 254 nm and the polyamines by fluorescence detection after post-column derivatization with o-phthalaldehyde. The method allows the determination of the SAM analogues and the polyamines in one single run by direct injection of tissue extracts. The procedure is applied to the study in rats and in hepatoma tissue culture cells of the biochemical effects of alpha difluoromethylornithine, a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase.

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Year:  1982        PMID： 6801066     DOI： 10.1016/s0378-4347(00)80389-8

Source DB:  PubMed          Journal:  J Chromatogr


  18 in total

1.  Uptake of the antitrypanosomal drug 5'-([(Z)-4-amino-2-butenyl]methylamino)-5'-deoxyadenosine (MDL 73811) by the purine transport system of Trypanosoma brucei brucei.

Authors:  T L Byers; P Casara; A J Bitonti
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

Review 2.  Recent advances in the biochemistry of polyamines in eukaryotes.

Authors:  A E Pegg
Journal:  Biochem J       Date:  1986-03-01       Impact factor: 3.857

3.  Polyamine depletion increases cellular ribonucleotide levels.

Authors:  S M Oredsson; M Kanje; P S Mamont; J Wagner; O Heby
Journal:  Mol Cell Biochem       Date:  1986-04       Impact factor: 3.396

4.  Characterization of Trypanosoma brucei brucei S-adenosyl-L-methionine decarboxylase and its inhibition by Berenil, pentamidine and methylglyoxal bis(guanylhydrazone).

Authors:  A J Bitonti; J A Dumont; P P McCann
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

5.  Antitrypanosomal effects of polyamine biosynthesis inhibitors correlate with increases in Trypanosoma brucei brucei S-adenosyl-L-methionine.

Authors:  T L Byers; T L Bush; P P McCann; A J Bitonti
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857

6.  The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli.

Authors:  A Bergerat; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

7.  Decreased protein-synthetic activity is an early consequence of spermidine depletion in rat hepatoma tissue-culture cells.

Authors:  B B Rudkin; P S Mamont; N Seiler
Journal:  Biochem J       Date:  1984-02-01       Impact factor: 3.857

8.  Sites of synthesis, translocation and accumulation of pyrrolizidine alkaloid N-oxides in Senecio vulgaris L.

Authors:  T Hartmann; A Ehmke; U Eilert; K von Borstel; C Theuring
Journal:  Planta       Date:  1989-01       Impact factor: 4.116

9.  Effects of selenomethionine on cell growth and on S-adenosylmethionine metabolism in cultured malignant cells.

Authors:  E O Kajander; R J Harvima; L Kauppinen; K K Akerman; H Martikainen; R L Pajula; S O Kärenlampi
Journal:  Biochem J       Date:  1990-05-01       Impact factor: 3.857

10.  The presence of an active S-adenosylmethionine decarboxylase gene increases the growth defect observed in Saccharomyces cerevisiae mutants unable to synthesize putrescine, spermidine, and spermine.

Authors:  D Balasundaram; Q W Xie; C W Tabor; H Tabor
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490
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