Origin of malignant adenocarcinoma cell line induced by retrovirus-like particles from DMBA rat mammary tumors.

Intraperitoneal inoculation of neonate Sprague Dawley rats with cell-free extracts containing retrovirus-like particles from DMBA-induced rat mammary tumors resulted in a fivefold increase of benign mammary neoplasias in the survivor animals, in comparison to the spontaneous tumor incidence rate. In addition, four animals developed metastasizing abdominal adenocarcinomas. The ascitic cells of one of the abdominal tumors were established as a permanent tissue culture line (HH-1). After subsequent animal passage, cells of the permanent line HH-9 clone 14 showed increased malignancy manifested by the number of takes per animals injected, and by the number of remote metastases observed.