Literature DB >> 6800272

Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF).

M A Donets, G V Rezapkin, A P Ivanov, E A Tkachenko.   

Abstract

Utilization of direct and indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) for the diagnosis of Crimean-Congo hemorrhagic fever (CCHF) allows the detection of low amounts of infectious virus (2 log LD50) or inactivated antigen and antibody to CCHF within 5-6 hours. These methods were shown to be more sensitive, specific, rapid, and reproducible than the complement-fixation test, immunofluorescence, hemagglutination, or radial diffusion in gel. The experimental design of ELISA and SPRIA developed for CCHF may be used successfully for the detection of the other members of the Bunyaviridae family.

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Year:  1982        PMID: 6800272     DOI: 10.4269/ajtmh.1982.31.156

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN: 0002-9637            Impact factor:   2.345


  7 in total

1.  Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus.

Authors:  A J Shepherd; R Swanepoel; P A Leman; S P Shepherd
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

2.  Enzyme-linked immunosorbent assays for the detection of antibody to Crimean-Congo haemorrhagic fever virus in the sera of livestock and wild vertebrates.

Authors:  F J Burt; R Swanepoel; L E Braack
Journal:  Epidemiol Infect       Date:  1993-12       Impact factor: 2.451

3.  Serodiagnosis of Crimean-Congo haemorrhagic fever.

Authors:  F J Burt; P A Leman; J C Abbott; R Swanepoel
Journal:  Epidemiol Infect       Date:  1994-12       Impact factor: 2.451

4.  Evaluation of enzyme-linked immunosorbent assay and reversed passive hemagglutination for detection of Crimean-Congo hemorrhagic fever virus antigen.

Authors:  A J Shepherd; R Swanepoel; D E Gill
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

5.  Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus.

Authors:  Nurettin Canakoglu; Engin Berber; Mustafa Ertek; Mustafa D Yoruk; Sukru Tonbak; Yusuf Bolat; Munir Aktas; Ahmet Kalkan; Aykut Ozdarendeli
Journal:  Virol J       Date:  2013-01-03       Impact factor: 4.099

6.  Imported Crimean-Congo hemorrhagic fever cases in Istanbul.

Authors:  Kenan Midilli; Ayşen Gargili; Onder Ergonul; Gönül Sengöz; Recep Ozturk; Mehmet Bakar; Frans Jongejan
Journal:  BMC Infect Dis       Date:  2007-06-06       Impact factor: 3.090

Review 7.  Seroepidemiological Studies of Crimean-Congo Hemorrhagic Fever Virus in Domestic and Wild Animals.

Authors:  Jessica R Spengler; Éric Bergeron; Pierre E Rollin
Journal:  PLoS Negl Trop Dis       Date:  2016-01-07
  7 in total

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