Literature DB >> 679954

Electronic platelet counting with capillary blood samples--experiences in a paediatric clinic.

H Gadner, E Odenwald, H Riehm, W H Hitzig.   

Abstract

For several reasons, electronic platelet counts have not been generally adopted in paediatric routine laboratories. A method of platelet counting in capillary blood samples using an electronic counter is described. This is based on brief centrifugation of the blood sample after the addition of a diluent containing heparin/dextran and with a density of 1.050. The thrombocytes are uniformly suspended in the supernatant after 5 min of centrifugation at 250 g, so that the counting procedure in the electric unit can follow immediately. This simplified, dependable and reproducible method is also suitable for venous blood, and as a result of several years of successful trial, it can be recommended for routine use, especially in clinics with paediatric patients.

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Year:  1978        PMID: 679954     DOI: 10.1007/BF00441372

Source DB:  PubMed          Journal:  Eur J Pediatr        ISSN: 0340-6199            Impact factor:   3.183


  19 in total

1.  [Manual and mechanized counter technics for thrombocytes determination].

Authors:  U Höffler; K Oette
Journal:  Med Lab (Stuttg)       Date:  1975-01

2.  SIMPLE METHOD FOR SEPARATING PLATELETS FROM RED CELLS BEFORE ENUMERATION WITH AN ELECTRONIC COUNTER.

Authors:  R D EASTHAM
Journal:  J Clin Pathol       Date:  1965-03       Impact factor: 3.411

3.  Separation of platelets from whole blood by the use of silicone liquids.

Authors:  M C MORGAN; J J SZAFIR
Journal:  Blood       Date:  1961-06       Impact factor: 22.113

4.  The reproducibility and constancy of the platelet count.

Authors:  G BRECHER; M SCHNEIDERMAN; E P CRONKITE
Journal:  Am J Clin Pathol       Date:  1953-01       Impact factor: 2.493

5.  Platelet counting using the Coulter electronic counter.

Authors:  M J Eggleton; A A Sharp
Journal:  J Clin Pathol       Date:  1963-03       Impact factor: 3.411

6.  Separation and purification of leukocytes and platelets.

Authors:  J L TULLIS
Journal:  Blood       Date:  1952-09       Impact factor: 22.113

7.  Methods for separating platelets from blood and plasma.

Authors:  H J Day; H Holmsen; M B Zucker
Journal:  Thromb Diath Haemorrh       Date:  1975-06-30

8.  Improved method for electronic counting of platelets.

Authors:  G L Fry; J C Hoak
Journal:  J Lab Clin Med       Date:  1969-09

9.  In vitro clumping of human platelets by dextran.

Authors:  D P Dhall; N A Matheson
Journal:  Thromb Diath Haemorrh       Date:  1968-03-31

10.  Automatic platelet counting with the Couler particle counter.

Authors:  R E Davis; R E Green
Journal:  J Clin Pathol       Date:  1967-09       Impact factor: 3.411

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