Literature DB >> 6799294

Purification and chemical properties of two 1,3;1,4-beta-glucan endohydrolases from germinating barley.

J R Woodward, G B Fincher.   

Abstract

Two 1,3;1,4-beta-glucan endohydrolases have been purified from extracts of germinating barley by ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. Both enzymes are monomeric, basic proteins. Enzyme I has a molecular weight of 28000 and an isoelectric point of 8.5, while enzyme II has a molecular weight of 33000 and an isoelectric point greater than 10. Enzyme II is a glycoprotein containing 3.6% carbohydrate, of which three residues are probable N-acetylglucosamine, but enzyme I contains only traces of associated carbohydrate. The amino acid compositions of the two 1,3;1,4-beta-glucan endohydrolases are similar and the cross-reactivity of antibodies raised against the purified enzymes suggests that they share common antigenic determinants.

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Year:  1982        PMID： 6799294     DOI： 10.1111/j.1432-1033.1982.tb05837.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  22 in total

1.  Structure and tissue-specific regulation of genes encoding barley (1----3, 1----4)-beta-glucan endohydrolases.

Authors:  N Slakeski; D C Baulcombe; K M Devos; B Ahluwalia; D N Doan; G B Fincher
Journal:  Mol Gen Genet       Date:  1990-12

2.  Purification of (1-->3)-beta-glucan endohydrolase isoenzyme II from germinated barley and determination of its primary structure from a cDNA clone.

Authors:  P B Høj; D J Hartman; N A Morrice; D N Doan; G B Fincher
Journal:  Plant Mol Biol       Date:  1989-07       Impact factor: 4.076

3.  Purification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point alpha-glucosidase from barley malt.

Authors:  T P Frandsen; F Lok; E Mirgorodskaya; P Roepstorff; B Svensson
Journal:  Plant Physiol       Date:  2000-05       Impact factor: 8.340

4.  Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase.

Authors:  Maria Hrmova; Rachel A Burton; Peter Biely; Jelle Lahnstein; Geoffrey B Fincher
Journal:  Biochem J       Date:  2006-10-01       Impact factor: 3.857

5.  Developmental Regulation of (1-->3, 1-->4)-beta-Glucanase Gene Expression in Barley : Tissue-Specific Expression of Individual Isoenzymes.

Authors:  N Slakeski; G B Fincher
Journal:  Plant Physiol       Date:  1992-07       Impact factor: 8.340

Review 6.  Structure-function relationships of beta-D-glucan endo- and exohydrolases from higher plants.

Authors:  M Hrmova; G B Fincher
Journal:  Plant Mol Biol       Date:  2001-09       Impact factor: 4.076

7.  Structure and expression of a barley acidic beta-glucanase gene.

Authors:  D E Malehorn; K J Scott; D M Shah
Journal:  Plant Mol Biol       Date:  1993-05       Impact factor: 4.076

8.  Three-dimensional structures of two plant beta-glucan endohydrolases with distinct substrate specificities.

Authors:  J N Varghese; T P Garrett; P M Colman; L Chen; P B Høj; G B Fincher
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-29       Impact factor: 11.205

9.  Messenger RNAs from the Scutellum and Aleurone of Germinating Barley Encode (1-->3,1-->4)-beta-d-Glucanase, alpha-Amylase and Carboxypeptidase.

Authors:  J Mundy; A Brandt; G B Fincher
Journal:  Plant Physiol       Date:  1985-11       Impact factor: 8.340

10.  Purification and characterization of (1-->3, 1-->4)-beta-glucan endohydrolases from germinated wheat (Triticum aestivum).

Authors:  D M Lai; P B Høj; G B Fincher
Journal:  Plant Mol Biol       Date:  1993-08       Impact factor: 4.076
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