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Literature DB >> 6798931

Bacillus subtilis "rec assay" test with isogenic strains.

Abstract

The Bacillus subtilis "rec assay" test, widely used for the detection of DNA-damaging agents, was studied in detail, using an isogenic set of strains carrying different mutations in repair or recombination functions or both. recE4 and rec-45 mutations turned out to confer on the cells the highest sensitivity to known mutagens. The recE4 strain and its isogenic rec+ strain have been tested and validated by several rec assay procedures for preferential killing by known DNA-damaging agents. The use of purified spores of the tester strains offers advantages for standardization of the assay.

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Year: 1982 PMID： 6798931 PMCID： PMC241798 DOI： 10.1128/aem.43.1.177-184.1982

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

18 in total

1. Mutagenicity of metronidazole: activation by mammalian liver microsomes.

Authors: H S Rosenkranz; W T Speck
Journal: Biochem Biophys Res Commun Date: 1975-09-16 Impact factor: 3.575

2. Screening of pesticides for DNA interactions by "rec-assay" and mutagenesis testing, and frameshift mutagens detected.

Authors: T Kada; M Moriya; Y Shirasu
Journal: Mutat Res Date: 1974-08 Impact factor: 2.433

3. An improved bacterial test system for the detection and classification of mutagens and carcinogens.

Authors: B N Ames; F D Lee; W E Durston
Journal: Proc Natl Acad Sci U S A Date: 1973-03 Impact factor: 11.205

4. In vitro and host-mediated "rec-assay" procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected.

Authors: T Kada; K Tutikawa; Y Sadaie
Journal: Mutat Res Date: 1972-10 Impact factor: 2.433

5. Rapid detection of mutagens and carcinogens.

Authors: E E Slater; M D Anderson; H S Rosenkranz
Journal: Cancer Res Date: 1971-07 Impact factor: 12.701

6. Induction of closely linked multiple mutations by nitrosoguanidine.

Authors: N Guerola; J L Ingraham; E Cerdá-Olmedo
Journal: Nat New Biol Date: 1971-03-24

7. Synchronous germination and outgrowth of fractionated Bacillus subtilis spores: tool for the analysis of differentiation and division of bacterial cells.

Authors: A G Siccardi; A Galizzi; G Mazza; A Clivio; A M Albertini
Journal: J Bacteriol Date: 1975-01 Impact factor: 3.490

5. Mutagenic and membranal effect of a phytotoxic molecule isolated from olive leaves parasitized by the fungus Cycloconium oleaginum Cast.

Authors: R Yahiaoui; A Guechi; E Lukasova; L Girre
Journal: Mycopathologia Date: 1994-05 Impact factor: 2.574

5 in total

Bacillus subtilis "rec assay" test with isogenic strains.

1. Mutagenicity of metronidazole: activation by mammalian liver microsomes.

2. Screening of pesticides for DNA interactions by "rec-assay" and mutagenesis testing, and frameshift mutagens detected.

3. An improved bacterial test system for the detection and classification of mutagens and carcinogens.

4. In vitro and host-mediated "rec-assay" procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected.

5. Rapid detection of mutagens and carcinogens.

6. Induction of closely linked multiple mutations by nitrosoguanidine.

7. Synchronous germination and outgrowth of fractionated Bacillus subtilis spores: tool for the analysis of differentiation and division of bacterial cells.

8. Activation of nitrofurantoin to a mutagen by rat liver nitroreductase.

9. Recombination-deficient mutants of Bacillus subtilis.

10. Mutagenicity screening of pesticides in the microbial system.

1. Mechanism of the Heat Sensitization of Bacillus subtilis Spores by Ethidium Bromide.

2. Molecular cloning of Bacillus subtilis genes involved in DNA metabolism.

3. Rapid assay for detection of microorganisms producing DNA-damaging metabolites.

4. Heat and UV light resistance of vegetative cells and spores of Bacillus subtilis Rec-mutants.

5. Mutagenic and membranal effect of a phytotoxic molecule isolated from olive leaves parasitized by the fungus Cycloconium oleaginum Cast.