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Literature DB >> 6797405

A requirement for EDTA in the separation of Photosystems 1 and 2 from the cyanobacterium Chlorogloea fritschii.

Abstract

Fractions enriched in Photosystem 1 or Photosystem 2 activity have been isolated from the cyanobacterium Chlorogloea fritschii after extraction of the membranes with digitonin and Triton X-100. Separation of the extract into the two components was achieved by using a Sepharose 6B column, calibration of which gave Kd values of 0.3 for the Photosystem 1 fraction and 0.53 for Photosystem 2. These values corresponded to molecular weights of approx. 500000 and 90000 respectively. The Photosystem 1 particle was shown to aggregate on storage and EDTA was shown to be necessary to separate the Photosystem 1 and 2 fractions.

Entities: Chemical Species

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Year: 1981 PMID： 6797405 PMCID： PMC1163020 DOI： 10.1042/bj1960489

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

10 in total

1. The electrophoretic isolation and partial characterization of three chlorophyll-protein complexes from blue-green algae.

Authors: S Reinman; J P Thornber
Journal: Biochim Biophys Acta Date: 1979-08-14

2. Detection of allophycocyanin in photosystem I preparations from the blue--green alga, Chlorogloea fritschii.

Authors: C A Pullin; R G Brown; E H Evans
Journal: FEBS Lett Date: 1979-05-01 Impact factor: 4.124

3. Mössbauer spectra of photosystem-I-reaction centres from the blue-green alga Chlorogloea fritschii.

Authors: E H Evans; J D Rush; C E Johnson; M C Evans
Journal: Biochem J Date: 1979-09-15 Impact factor: 3.857

4. Photoreduction of 2,6-dichlorophenolindophenol by diphenylcarbazide: a photosystem 2 reaction catalyzed by tris-washed chloroplasts and subchloroplast fragments.

Authors: L P Vernon; E R Shaw
Journal: Plant Physiol Date: 1969-11 Impact factor: 8.340

10. Isolation and characterization of photosystem I and II membrane particles from the blue-green alga, Synechococcus cedrorum.

Authors: P J Newman; L A Sherman
Journal: Biochim Biophys Acta Date: 1978-08-08

10 in total

1 in total

Review 1. Respiration and photosynthesis in energy-transducing membranes of cyanobacteria.

Authors: A Binder
Journal: J Bioenerg Biomembr Date: 1982-12 Impact factor: 2.945

1 in total

A requirement for EDTA in the separation of Photosystems 1 and 2 from the cyanobacterium Chlorogloea fritschii.

1. The electrophoretic isolation and partial characterization of three chlorophyll-protein complexes from blue-green algae.

2. Detection of allophycocyanin in photosystem I preparations from the blue--green alga, Chlorogloea fritschii.

3. Mössbauer spectra of photosystem-I-reaction centres from the blue-green alga Chlorogloea fritschii.

4. Photoreduction of 2,6-dichlorophenolindophenol by diphenylcarbazide: a photosystem 2 reaction catalyzed by tris-washed chloroplasts and subchloroplast fragments.

Review 5. The primary electron acceptor of photosystem. I.

6. Properties and structure of fractions prepared from Anabaena variabilis by the action Triton X-100.

7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

8. Purification and properties of the photosystem I reaction center from chloroplasts.

9. Composition of a photosystem I chlorophyll protein complex from Anabaena flos-aquae.

10. Isolation and characterization of photosystem I and II membrane particles from the blue-green alga, Synechococcus cedrorum.

Review 1. Respiration and photosynthesis in energy-transducing membranes of cyanobacteria.