Literature DB >> 6794638

Isolation and characterization of a soluble, immunoactive peptide of glial fibrillary acidic protein.

B S Hong, P F Davison.   

Abstract

A soluble immunoactive peptide with a molecular weight of 16 000 was isolated and purified from the cyanogen bromide digest of the insoluble 50 000 dalton glial fibrillary acidic protein by Sephacryl S-200 gel filtration followed by DEAE-Bio-gel A chromatography. The homogeneity of the peptide was established by SDS-polyacrylamide gel electrohporesis and isoelectric focusing. The peptide from several species showed immunocrossreaction with rabbit antibody to intact glial fibrillary acidic protein. The peptide has a pI value of 5.32. The amino acid sequence of 28 residues from the amino terminus of the calf peptide has been determined.

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Year:  1981        PMID： 6794638     DOI： 10.1016/0005-2795(81)90001-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Masking of epitopes in tissue sections. A study of glial fibrillary acidic (GFA) protein with antisera and monoclonal antibodies.

Authors:  D Dahl; M Grossi; A Bignami
Journal:  Histochemistry       Date:  1984

2.  Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.

Authors:  S A Lewis; J M Balcarek; V Krek; M Shelanski; N J Cowan
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

3.  Intermediate filaments and anaplastic change of ENU-induced gliomass: immunohistochemical study with vimentin and astroprotein (GFAP).

Authors:  T Yoshimine; M Maruno; Y Ushio; T Hayakawa; Y Nakajima; H Mogami
Journal:  J Neurooncol       Date:  1987       Impact factor: 4.130

4.  The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.

Authors:  N Geisler; K Weber
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

5.  The structural relation between intermediate filament proteins in living cells and the alpha-keratins of sheep wool.

Authors:  K Weber; N Geisler
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

6.  Reconstitution of cytokeratin filaments in vitro: further evidence for the role of nonhelical peptides in filament assembly.

Authors:  J J Sauk; M Krumweide; D Cocking-Johnson; J G White
Journal:  J Cell Biol       Date:  1984-11       Impact factor: 10.539

7.  Amino acid sequence data on glial fibrillary acidic protein (GFA); implications for the subdivision of intermediate filaments into epithelial and non-epithelial members.

Authors:  N Geisler; K Weber
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  7 in total

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