Literature DB >> 6791621

An improved assay for interleukin 2 (lymphocyte growth factor) produced by mitogen-activated lymphocytes.

K J Lafferty, S J Prowse, A Al-Adra, H S Warren, J Vasalli, E Reich.   

Abstract

The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.

Entities:  

Mesh:

Substances:

Year:  1980        PMID: 6791621     DOI: 10.1038/icb.1980.55

Source DB:  PubMed          Journal:  Aust J Exp Biol Med Sci        ISSN: 0004-945X


  14 in total

1.  Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis.

Authors:  D N McMurray; C L Mintzer; R A Bartow; R L Parr
Journal:  Infect Immun       Date:  1989-09       Impact factor: 3.441

2.  Cloning and expression of the rat interleukin-3 gene.

Authors:  D R Cohen; A J Hapel; I G Young
Journal:  Nucleic Acids Res       Date:  1986-05-12       Impact factor: 16.971

3.  T-cell repopulation following neonatal injection of non-obese diabetic (NOD) mice with anti-T-cell antibodies.

Authors:  A Hayward; M Shriber; R Kubo; M McDuffie
Journal:  Immunology       Date:  1992-05       Impact factor: 7.397

4.  Production of lymphocyte activating factor in vivo.

Authors:  P R Wood; L Andrus; I A Clark
Journal:  Immunology       Date:  1983-12       Impact factor: 7.397

5.  Sensitization of mice with wild-type and cold-adapted influenza virus variants: immune response to two H1N1 and H3N2 viruses.

Authors:  S J Tao; N K Mak; G L Ada
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

6.  T cells can be cytotoxic without making interleukin 2: a model of separate pathways of induction.

Authors:  D Bellgrau; D W Talmage
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

7.  Suppression of interleukin-2 production by macrophages in genetically susceptible mice infected with Leishmania major.

Authors:  E Cillari; F Y Liew; R Lelchuk
Journal:  Infect Immun       Date:  1986-11       Impact factor: 3.441

8.  Production of human and murine interleukin-2 by toxic shock syndrome toxin-1.

Authors:  V V Micusan; G Mercier; A R Bhatti; R F Reiser; M S Bergdoll; D Oth
Journal:  Immunology       Date:  1986-06       Impact factor: 7.397

9.  Generation of interleukin-2-dependent T cell lines from synovial fluids in rheumatoid arthritis.

Authors:  J A Wilkins; S L Olivier; R J Warrington
Journal:  Clin Exp Immunol       Date:  1984-10       Impact factor: 4.330

10.  Traffic and proliferative responses of recirculating lymphocytes in fetal calves.

Authors:  W R Hein; J N Shelton; M W Simpson-Morgan; B Morris
Journal:  Immunology       Date:  1988-08       Impact factor: 7.397

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.