Literature DB >> 6788854

Use of enzyme-linked immunosorbent assays (ELISA) for detection of monoclonal antibodies: experience with antigens of Toxoplasma gondii.

Y Naot, J S Remington.   

Abstract

The usefulness of enzyme-linked immunosorbent assay (ELISA) systems for detection of monoclonal antibodies to Toxoplasma gondii was studied. Seven monoclonal antibodies with specificities for membrane antigens, cytoplasmic antigens, or both membrane and cytoplasmic antigens of T. gondii were tested in a "sandwich" or in a "double sandwich" assay. Whereas both ELISA systems were sensitive and specific for detection of monoclonal antibodies to T. gondii, the double sandwich ELISA proved more sensitive than did the sandwich assay. Using different T. gondii antigen preparations in both assays, we demonstrated that the ELISA systems are specific, sensitive, rapid, and easy to perform and are therefore useful for screening and detection of monoclonal antibodies of desired isotypes and defined specificities.

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Year:  1981        PMID： 6788854     DOI： 10.1016/0022-1759(81)90181-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens.

Authors:  T Chardès; I Bourguin; M N Mevelec; J F Dubremetz; D Bout
Journal:  Infect Immun       Date:  1990-05       Impact factor: 3.441

2.  Serodiagnosis of human G and M immunoglobulins to Toxoplasma gondii by ELISA using whole tachyzoites as antigens: a comparative study with the indirect haemagglutination (IHA) and immunofluorescence (IFA) tests.

Authors:  J P Tomasi; N Barka; S Stadtsbaeder
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402

3.  Comparison between ELISA and dye test for detection of naturally acquired toxoplasma gondii antibodies in the goat.

Authors:  B P Berdal; O Olsvik; T Almlid; H J Larsen; A M Lorentzen-Styr
Journal:  Acta Vet Scand       Date:  1983       Impact factor: 1.695

4.  Development of panel of monoclonal antibodies specific to urochordate cell surface antigens.

Authors:  Ziva Lapidot; Baruch Rinkevich
Journal:  Mar Biotechnol (NY)       Date:  2005-06-17       Impact factor: 3.619

5.  Monoclonal antibodies that inhibit mitogenic activity of Mycoplasma pulmonis.

Authors:  Z Lapidot; R Siman-Tov; Y Naot
Journal:  Infect Immun       Date:  1995-01       Impact factor: 3.441

6.  Ultrastructural localization of an intracellular Toxoplasma protein that induces protection in mice.

Authors:  L D Sibley; S D Sharma
Journal:  Infect Immun       Date:  1987-09       Impact factor: 3.441

7.  Immunoenzymatic detection of three kinds of 43,000-molecular-weight antigens by monoclonal antibodies in the insoluble fraction of Toxoplasma gondii.

Authors:  K Ogata; T Kasahara; K Shioiri-Nakano; I Igarashi; M Suzuki
Journal:  Infect Immun       Date:  1984-03       Impact factor: 3.441

8.  A monoclonal antibody with specificity for Trypanosoma cruzi, central and peripheral neurones and glia.

Authors:  D Snary; J E Flint; J N Wood; M T Scott; M D Chapman; J Dodd; T M Jessell; M A Miles
Journal:  Clin Exp Immunol       Date:  1983-12       Impact factor: 4.330

9.  Characterization of Toxoplasma gondii antigens that react with human immunoglobulin M and immunoglobulin G antibodies.

Authors:  Y Naot; D R Guptill; J Mullenax; J S Remington
Journal:  Infect Immun       Date:  1983-07       Impact factor: 3.441

10.  Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus.

Authors:  G Solomon; J Schiffenbauer; H D Keiser; B Diamond
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205
  10 in total

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