Literature DB >> 6788382

The association of rhodamine - labelled alpha-actinin with actin bundles in demembranated cells.

B Geiger.   

Abstract

Rhodamine-labelled alpha-actinin was specifically bound to actin containing filament bundles of demembranated fibroblasts, and was particularly associated with their termini. Optimal binding of rhodamine-alpha-actinin occurred at pH 6.0 - 6.2 and could be abolished by the addition of unlabeled alpha-actinin or myosin subfragment 1. The spatial relationships between the decorating alpha-actinin and several actin associated proteins was determined by double fluorescence microscopy.

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Year:  1981        PMID： 6788382     DOI： 10.1016/s0309-1651(81)80015-x

Source DB:  PubMed          Journal:  Cell Biol Int Rep        ISSN： 0309-1651


  8 in total

1.  A cell-free system to study regulation of focal adhesions and of the connected actin cytoskeleton.

Authors:  A Cattelino; C Albertinazzi; M Bossi; D R Critchley; I de Curtis
Journal:  Mol Biol Cell       Date:  1999-02       Impact factor: 4.138

2.  The localization of actin in dividing corneal endothelial cells demonstrated with nitrobenzoxadiazole phallacidin.

Authors:  S R Gordon
Journal:  Cell Tissue Res       Date:  1983       Impact factor: 5.249

3.  Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin.

Authors:  J M Sanger; B Mittal; M B Pochapin; J W Sanger
Journal:  J Cell Biol       Date:  1986-06       Impact factor: 10.539

4.  Interaction of fluorescently-labeled contractile proteins with the cytoskeleton in cell models.

Authors:  J W Sanger; B Mittal; J M Sanger
Journal:  J Cell Biol       Date:  1984-09       Impact factor: 10.539

5.  Microfilament-organizing centers in areas of cell contact: cytoskeletal interactions during cell attachment and locomotion.

Authors:  B Geiger; Z Avnur; G Rinnerthaler; H Hinssen; V J Small
Journal:  J Cell Biol       Date:  1984-07       Impact factor: 10.539

6.  Vinculin interaction with permeabilized cells: disruption and reconstitution of a binding site.

Authors:  E H Ball; C Freitag; S Gurofsky
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539

7.  Subcellular distribution of rhodamine-actin microinjected into living fibroblastic cells.

Authors:  S D Glacy
Journal:  J Cell Biol       Date:  1983-10       Impact factor: 10.539

8.  Analysis of myofibrillar structure and assembly using fluorescently labeled contractile proteins.

Authors:  J W Sanger; B Mittal; J M Sanger
Journal:  J Cell Biol       Date:  1984-03       Impact factor: 10.539
  8 in total

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