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Literature DB >> 678422

A soft-agar procedure measuring growth of human colonic carcinomas.

Abstract

Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

Entities: Chemical Disease Species

Mesh：

Substances：
Culture Media
Agar

Year: 1978 PMID： 678422 PMCID： PMC2009639 DOI： 10.1038/bjc.1978.147

Source DB: PubMed Journal: Br J Cancer ISSN： 0007-0920 Impact factor: 7.640

11 in total

A soft-agar procedure measuring growth of human colonic carcinomas.

1. A rapid method for establishing short-term primary cultures of human tumor cells from fresh tumor biopsies.

2. Cell fractionation of large bowel cancer.

3. Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.

4. beta-Hexosaminidase isozymes in human colonic carcinoma.

5. Classification of human colorectal adenocarcinoma cell lines.

6. The use of the Robinson in vitro agar culture assay in adult acute leukemia.

7. Growth kinetics of human large bowel cancer growing in immune-deprived mice and some chemotherapeutic observations.

8. Partial purification of human colonic carcinoma cells by sedimentation.

9. Ultrastructural analysis of hematopoietic colonies derived from human peripheral blood. A newly developed method.

10. A colony-forming assay for human tumour xenografts using agar in diffusion chambers.

Review 1. Heterogeneity of human colon carcinoma.

2. Cytotoxic activity of the thioether phospholipid analogue BM 41.440 in primary human tumor cultures.

3. Clonogenic growth and hormone sensitivity of benign and malignant breast tissues.

4. Antigenic heterogeneity and individuality in adenocarcinomas of the rectum and their secondaries.