Literature DB >> 6781490

Proteinase activity in chondroitin lyase (chondroitinase) and endo-beta-D-galactosidase (keratanase) preparations and a method to abolish their proteolytic effect on proteoglycan.

Y Oike, K Kimata, T Shinomura, S Suzuki.   

Abstract

Significant amounts of proteinase activity have been found in chondroitin ABC lyase (EC 4.2.2.4), chondroitin AC II lyase and endo-beta-D-galactosidase (keratanase) from commercial sources. It would appear, therefore, that certain earlier biochemical and histochemical studies, which employed these commercial enzyme preparations for their presumed ability to degrade only glycosaminoglycans, may require re-evaluation. A mixture of EDTA, N-ethylmaleimide, phenylmethanesulphonyl fluoride and pepstatin abolishes the effect of the contaminating proteinases on proteoglycan with less significant effect on the chondroitin lyase or keratanase activity.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6781490      PMCID： PMC1162198          DOI： 10.1042/bj1910203

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  A submicrodetermination of glucose.

Authors:  J T PARK; M J JOHNSON
Journal:  J Biol Chem       Date:  1949-11       Impact factor: 5.157

2.  Purification and properties of bacterial chondroitinases and chondrosulfatases.

Authors:  T Yamagata; H Saito; O Habuchi; S Suzuki
Journal:  J Biol Chem       Date:  1968-04-10       Impact factor: 5.157

3.  The occurrence of low buoyant density proteoglycans in embryonic chick cartilage.

Authors:  K Kimata; Y Oike; K Ito; K Karasawa; S Suzuki
Journal:  Biochem Biophys Res Commun       Date:  1978-12-29       Impact factor: 3.575

4.  Structural analysis of chick-embryo cartilage proteoglycan by selective degradation with chondroitin lyases (chondroitinases) and endo-beta-D-galactosidase (keratanase).

Authors:  Y Oike; K Kimata; T Shinomura; K Nakazawa; S Suzuki
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

5.  Effect of pepstatin on acid proteases.

Authors:  T Aoyagi; S Kunimoto; H Morishima; T Takeuchi; H Umezawa
Journal:  J Antibiot (Tokyo)       Date:  1971-10       Impact factor: 2.649

6.  Purification of Keratan Sulfate-endogalactosidase and its action on keratan sulfates of different origin.

Authors:  K Nakazawa; S Suzuki
Journal:  J Biol Chem       Date:  1975-02-10       Impact factor: 5.157

7.  Crystallization and some properties of chondroitinase from Arthrobacter aurescens.

Authors:  K Hiyama; S Okada
Journal:  J Biol Chem       Date:  1975-03-10       Impact factor: 5.157

8.  Sequential degradation of keratan sulfate by bacterial enzymes and purification of a sulfatase in the enzymatic system.

Authors:  K Nakazawa; N Suzuki; S Suzuki
Journal:  J Biol Chem       Date:  1975-02-10       Impact factor: 5.157

9.  Role of hyaluronic acid in the in vivo aggregation of cartilage proteoglycans.

Authors:  G Quintarelli; A Vocaturo; L Rodén; M Bellocci; L M Vassallo
Journal:  Connect Tissue Res       Date:  1978       Impact factor: 3.417

10.  Action of chondroitinases. I. The mode of action of two chondroitinase-AC preparations of different origin.

Authors:  K Hiyama; S Okada
Journal:  J Biochem       Date:  1976-12       Impact factor: 3.387
  10 in total
  17 in total

1.  Ultrastructural localisation of anionic sites at the dermo-epidermal junction in normal human skin.

Authors:  J Nanchahal; D J Riches
Journal:  J Anat       Date:  1991-06       Impact factor: 2.610

2.  Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.

Authors:  M Takagi; M Maeno; Y Takahashi; K Otsuka
Journal:  Histochem J       Date:  1992-02

3.  Turnover of proteoglycans in articular-cartilage cultures. Characterization of proteoglycans released into the medium.

Authors:  M A Campbell; C J Handley; S E D'Souza
Journal:  Biochem J       Date:  1989-04-01       Impact factor: 3.857

4.  Structural analysis of chick-embryo cartilage proteoglycan by selective degradation with chondroitin lyases (chondroitinases) and endo-beta-D-galactosidase (keratanase).

Authors:  Y Oike; K Kimata; T Shinomura; K Nakazawa; S Suzuki
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

5.  Nature and distribution of chondroitin sulphate and dermatan sulphate proteoglycans in rabbit alveolar bone.

Authors:  M Takagi; M Maeno; T Yamada; K Miyashita; K Otsuka
Journal:  Histochem J       Date:  1996-05

6.  The process of calcification during development of the rat tracheal cartilage characterized by distribution of alkaline phosphatase activity and immunolocalization of types I and II collagens and glycosaminoglycans of proteoglycans.

Authors:  Y Sasano; I Mizoguchi; M Furusawa; N Aiba; E Ohtani; Y Iwamatsu; M Kagayama
Journal:  Anat Embryol (Berl)       Date:  1993-07

7.  Immunohistochemical localization of glycosaminoglycans in experimental rat glioma models.

Authors:  H Nioka; K Matsumura; S Nakasu; J Handa
Journal:  J Neurooncol       Date:  1994       Impact factor: 4.130

8.  Characterization of proteoglycans synthesized by human adult glomerular mesangial cells in culture.

Authors:  G J Thomas; R M Mason; M Davies
Journal:  Biochem J       Date:  1991-07-01       Impact factor: 3.857

9.  Synthesis of 35S-labelled macromolecules by polymorphonuclear neutrophils. Evidence for the production of [35S]sulphite which can modify both endogenous and exogenous proteins.

Authors:  E E Gardiner; H C Robinson; A Sriratana; S S Mok; D A Lowther; C J Handley
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857

10.  Effects of cations on cartilage structure: swelling of growth plate and degradation of proteoglycans induced by chelators of divalent cations.

Authors:  R D Campo
Journal:  Calcif Tissue Int       Date:  1988-08       Impact factor: 4.333
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.