Literature DB >> 6780488

Optimal fixation conditions for the immunoperoxidase identification of human J chain from tissue sections.

K Käyhkö.   

Abstract

J chain can be used as a marker of plasmablasts and plasma cells at an earlier stage than intracellular immunoglobulin. Immunoperoxidase techniques were used to study optimal fixation conditions for the preservation of human J chain antigenicity in paraffin-embedded tissue sections. The most constantly positive staining for J chain combined with good morphological integrity was obtained with Bouin's fluid for 1.5 h at 20 degrees C. All other fixatives studied showed less consistent staining results.

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Year:  1980        PMID: 6780488     DOI: 10.1007/BF00508842

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  11 in total

1.  Ultrastructural localization of J chain in human intestinal mucosa.

Authors:  H Nagura; P Brandtzaeg; P K Nakane; W R Brown
Journal:  J Immunol       Date:  1979-09       Impact factor: 5.422

2.  Enzyme-labeled antibodies: preparation and application for the localization of antigens.

Authors:  P K Nakane; G B Pierce
Journal:  J Histochem Cytochem       Date:  1966-12       Impact factor: 2.479

3.  Immunoperoxidase identification of intracellular immunoglobulins in human tissues.

Authors:  S Reitamo; J J Reitamo
Journal:  Am J Clin Pathol       Date:  1978-11       Impact factor: 2.493

4.  Site of J chain attachment to human polymeric IgA.

Authors:  J Mestecky; R E Schrohenloher; R Kulhavy; G P Wright; M Tomana
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205

5.  Structure and function of the J chain.

Authors:  M E Koshland
Journal:  Adv Immunol       Date:  1975       Impact factor: 3.543

6.  Presence of J chain in human immunocytes containing various immunoglobulin classes.

Authors:  P Brandtzaeg
Journal:  Nature       Date:  1974-11-29       Impact factor: 49.962

7.  Anomolous staining patterns in immunohistologic studies of malignant lymphoma.

Authors:  P Isaacson; D H Wright
Journal:  J Histochem Cytochem       Date:  1979-08       Impact factor: 2.479

8.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

9.  Immunochemical demonstration of J chain: a marker of B-cell malignancy.

Authors:  P Isaacson
Journal:  J Clin Pathol       Date:  1979-08       Impact factor: 3.411

10.  Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen-stimulated normal cells and in lymphoblastoid cell lines.

Authors:  J Mestecky; R J Winchester; T Hoffman; H G Kunkel
Journal:  J Exp Med       Date:  1977-03-01       Impact factor: 14.307

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