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Literature DB >> 6779812

A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid.

Abstract

A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.

Entities: Chemical Species

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Year: 1980 PMID： 6779812 PMCID： PMC1162008 DOI： 10.1042/bj1890369

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

6 in total

1. A highly sensitive and rapid fluorometric assay for UDP-glucuronyltransferase using 3-hydroxybenzo (a) pyrene as substrate.

Authors: J Singh; F J Wiebel
Journal: Anal Biochem Date: 1979-10-01 Impact factor: 3.365

2. Assay of UDPG1cUA pyrophosphatase and its relation to transglucuronidation.

Authors: K P Wong; Y K Lau
Journal: Biochim Biophys Acta Date: 1970-10-14

3. Measurement of nanogram quantities of UDP-glucuronic acid in tissues.

Authors: K P Wong
Journal: Anal Biochem Date: 1977-10 Impact factor: 3.365

4. Aryl hydrocarbon (benzo(a)pyrene) hydroxylase in microsomes from rat tissues: differential inhibition and stimulation by benzoflavones and organic solvents.

Authors: F J Wiebel; J C Leutz; L Diamond; H V Gelboin
Journal: Arch Biochem Biophys Date: 1971-05 Impact factor: 4.013

5. [Radioenzymatic micromethod for the determination of UDP-glucuronic acid (author's transl)].

Authors: W Lehnert; T O Wagner; M W Fürst; W Künzer
Journal: J Clin Chem Clin Biochem Date: 1979-06

6. UDP-glucuronyltransferase in perfused rat liver and in microsomes. Effects of CCl4 injury.

Authors: K W Bock; E Huber; W Schlote
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1977-02 Impact factor: 3.000

6 in total

5 in total

1. New methods to assess 6-thiopurine toxicity and expanding its therapeutic application to pancreatic cancer via small molecule potentiators.

Authors: Chamitha Weeramange; Ashabha Lansakara; Johnathan Dallman; Thi Nguyen; Wasundara Hulangamuwa; Ryan J Rafferty
Journal: Medchemcomm Date: 2019-03-18 Impact factor: 3.597

A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid.

1. A highly sensitive and rapid fluorometric assay for UDP-glucuronyltransferase using 3-hydroxybenzo (a) pyrene as substrate.

2. Assay of UDPG1cUA pyrophosphatase and its relation to transglucuronidation.

3. Measurement of nanogram quantities of UDP-glucuronic acid in tissues.

4. Aryl hydrocarbon (benzo(a)pyrene) hydroxylase in microsomes from rat tissues: differential inhibition and stimulation by benzoflavones and organic solvents.

5. [Radioenzymatic micromethod for the determination of UDP-glucuronic acid (author's transl)].

6. UDP-glucuronyltransferase in perfused rat liver and in microsomes. Effects of CCl4 injury.

1. New methods to assess 6-thiopurine toxicity and expanding its therapeutic application to pancreatic cancer via small molecule potentiators.

2. UDP-sugar metabolism in Swarm rat chondrosarcoma chondrocytes.

3. Altered disposition of acetaminophen in Nrf2-null and Keap1-knockdown mice.

4. Metabolism of heme and bilirubin in rat and human small intestinal mucosa.

5. Sex Is a Determinant for Deoxynivalenol Metabolism and Elimination in the Mouse.