Literature DB >> 677858

Iodometric assay method for beta-lactamase with various beta-lactam antibiotics as substrates.

T Sawai, I Takahashi, S Yamagishi.   

Abstract

The rapid fixed-time assay for penicillinase was modified for measuring beta-lactamase activity with twelve substrates, i.e., benzylpenicillin, ampicillin, cloxacillin, methicillin, carbenicillin, cefazolin, cephalothin, cephaloglycin, cephalexin, cephalosporin C, 7-aminocephalosporanic acid, and cefoxitin. The method depends upon the reduction of iodine by the hydrolyzed substrate. Determined experimentally, 1 mol of hydrolyzed penicillins consumed 3.4 to 4.0 mol of iodine (I(2)). Iodine consumption of hydrolyzed cephalosporins varied widely from 1.7 for cephalothin to 3.7 for cefazolin. The method is useful for routine assay of beta-lactamase activity with various substrates.

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Year:  1978        PMID： 677858      PMCID： PMC352361          DOI： 10.1128/AAC.13.6.910

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  6 in total

1.  Iodometric assay of penicillinase.

Authors:  C J PERRET
Journal:  Nature       Date:  1954-11-27       Impact factor: 49.962

2.  Thermolabile repression of cephalosporinase synthesis in Citrobacter freundii.

Authors:  T Sawai; S Nakajima; T Morohoshi; S Yamagishi
Journal:  Microbiol Immunol       Date:  1977-11       Impact factor: 1.955

3.  Variant of penicillinase mediated by an R factor in Escherichia coli.

Authors:  T Sawai; K Takahashi; S Yamagishi; S Mitsuhashi
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490

4.  Rapid fixed-time assay for penicillinase.

Authors:  M G Sargent
Journal:  J Bacteriol       Date:  1968-04       Impact factor: 3.490

5.  The purification and properties of penicillin beta-lactamases mediated by transmissible R factors in Escherichia coli.

Authors:  S Yamagishi; K O'Hara; T Sawai; S Mitsuhashi
Journal:  J Biochem       Date:  1969-07       Impact factor: 3.387

6.  Inducible oxacillin-hydrolyzing penicillinase in Aeromonas hydrophila isolated from fish.

Authors:  T Sawai; K Morioka; M Ogawa; S Yamagishi
Journal:  Antimicrob Agents Chemother       Date:  1976-08       Impact factor: 5.191
  6 in total
  30 in total

1.  Synthesis of Escherichia coli heat-stable enterotoxin STp as a pre-pro form and role of the pro sequence in secretion.

Authors:  K Okamoto; M Takahara
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

2.  Evaluation of the BYG Carba Test, a New Electrochemical Assay for Rapid Laboratory Detection of Carbapenemase-Producing Enterobacteriaceae.

Authors:  Pierre Bogaerts; Sami Yunus; Marion Massart; Te-Din Huang; Youri Glupczynski
Journal:  J Clin Microbiol       Date:  2015-12-04       Impact factor: 5.948

3.  Probable detection of kil peptide derived from colicin E1 plasmid in the envelope fraction of Escherichia coli HB101 carrying pEAP31.

Authors:  R Aono
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

4.  Structure and Functional Characterisation of a Distinctive β-Lactamase from an Environmental Strain EMB20 of Bacillus cereus.

Authors:  Ayesha Sadaf; Rajeshwari Sinha; S K Khare
Journal:  Appl Biochem Biotechnol       Date:  2017-06-29       Impact factor: 2.926

5.  Conjugative Transfer of IncP-9 Catabolic Plasmids Requires a Previously Uncharacterized Gene, mpfK, Whose Homologs Are Conserved in Various MPFT-Type Plasmids.

Authors:  Kouhei Kishida; Shouta Nonoyama; Tim Lukas; Shotaro Kawahara; Koji Kudo; Yuji Nagata; Yoshiyuki Ohtsubo; Masataka Tsuda
Journal:  Appl Environ Microbiol       Date:  2019-11-27       Impact factor: 4.792

6.  Isolation of Serratia marcescens mutants which could overproduce and excrete Escherichia coli alkaline phosphatase and beta-lactamase.

Authors:  S Asdornnithee; K Akiyama; R Takata
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

7.  Structural Basis of Reduced Susceptibility to Ceftazidime-Avibactam and Cefiderocol in Enterobacter cloacae Due to AmpC R2 Loop Deletion.

Authors:  Akito Kawai; Christi L McElheny; Alina Iovleva; Ellen G Kline; Nicolas Sluis-Cremer; Ryan K Shields; Yohei Doi
Journal:  Antimicrob Agents Chemother       Date:  2020-06-23       Impact factor: 5.191

8.  Ro 13-9904, a long-acting broad-spectrum cephalosporin: in vitro and in vivo studies.

Authors:  P Angehrn; P J Probst; R Reiner; R L Then
Journal:  Antimicrob Agents Chemother       Date:  1980-12       Impact factor: 5.191

9.  Characteristics of beta-lactamase-inhibiting proteins from Streptomyces exfoliatus SMF19.

Authors:  M K Kim; K J Lee
Journal:  Appl Environ Microbiol       Date:  1994-03       Impact factor: 4.792

10.  Characteristics of the endoglucanase encoded by a cel gene from Bacteroides succinogenes expressed in Escherichia coli.

Authors:  K A Taylor; B Crosby; M McGavin; C W Forsberg; D Y Thomas
Journal:  Appl Environ Microbiol       Date:  1987-01       Impact factor: 4.792
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