Literature DB >> 6777631

Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster.

A Krämer, R Haars, R Kabisch, H Will, F A Bautz, E K Bautz.   

Abstract

Monoclonal antibodies were raised against purified RNA polymerase II ( or B) from Drosophila melanogaster. The antibody produced by one hybridoma cell clone was found to be directed against the two large subunits of the enzyme. The absence of antibodies directed against proteins possibly contaminating the antigens used for immunization allowed us to identify RNA polymerase unequivocally in interbands and puffs of polytene chromosomes. Within a single heat shock puff (87C1) RNA polymerase was found to be clustered in two separate areas suggesting two distinct regions of RNA polymerase activity in this puff.

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Year:  1980        PMID： 6777631     DOI： 10.1007/bf00267369

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

1.  SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors:  J W LITTLEFIELD
Journal:  Science       Date:  1964-08-14       Impact factor: 47.728

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Messenger RNA in heat-shocked Drosophila cells.

Authors:  A Spradling; M L Pardue; S Penman
Journal:  J Mol Biol       Date:  1977-02-05       Impact factor: 5.469

6.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

7.  Antigenic homology of eukaryotic RNA polymerases.

Authors:  C J Ingles
Journal:  Biochem Biophys Res Commun       Date:  1973-11-16       Impact factor: 3.575

8.  A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster.

Authors:  J T Lis; L Prestidge; D S Hogness
Journal:  Cell       Date:  1978-08       Impact factor: 41.582

9.  Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells.

Authors:  A Spradling; S Penman; M L Pardue
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

10.  Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster.

Authors:  M Jamrich; A L Greenleaf; E K Bautz
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205
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  37 in total

1.  RNA polymerase II holoenzyme modifications accompany transcription reprogramming in herpes simplex virus type 1-infected cells.

Authors:  H L Jenkins; C A Spencer
Journal:  J Virol       Date:  2001-10       Impact factor: 5.103

2.  The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.

Authors:  Y G Gangloff; J C Pointud; S Thuault; L Carré; C Romier; S Muratoglu; M Brand; L Tora; J L Couderc; I Davidson
Journal:  Mol Cell Biol       Date:  2001-08       Impact factor: 4.272

3.  Nuclear translocation and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos.

Authors:  S Bellier; S Chastant; P Adenot; M Vincent; J P Renard; O Bensaude
Journal:  EMBO J       Date:  1997-10-15       Impact factor: 11.598

4.  The nuclear matrix protein p255 is a highly phosphorylated form of RNA polymerase II largest subunit which associates with spliceosomes.

Authors:  M Vincent; P Lauriault; M F Dubois; S Lavoie; O Bensaude; B Chabot
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

5.  Phosphorylation of the RNA polymerase II largest subunit during Xenopus laevis oocyte maturation.

Authors:  S Bellier; M F Dubois; E Nishida; G Almouzni; O Bensaude
Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

6.  Heat-shock inactivation of the TFIIH-associated kinase and change in the phosphorylation sites on the C-terminal domain of RNA polymerase II.

Authors:  M F Dubois; M Vincent; M Vigneron; J Adamczewski; J M Egly; O Bensaude
Journal:  Nucleic Acids Res       Date:  1997-02-15       Impact factor: 16.971

7.  Ecdysteroids regulate the synthesis of RNA polymerase molecules in fat body cells ofCalliphora larvae.

Authors:  Heide Schenkel; Klaus Scheller
Journal:  Wilehm Roux Arch Dev Biol       Date:  1983-05

8.  Dominant-negative mutants reveal a role for the Cdk7 kinase at the mid-blastula transition in Drosophila embryos.

Authors:  V Leclerc; S Raisin; P Léopold
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

9.  Herpes simplex virus type 1 infection leads to loss of serine-2 phosphorylation on the carboxyl-terminal domain of RNA polymerase II.

Authors:  Kathryn A Fraser; Stephen A Rice
Journal:  J Virol       Date:  2005-09       Impact factor: 5.103

10.  ICP27 interacts with the C-terminal domain of RNA polymerase II and facilitates its recruitment to herpes simplex virus 1 transcription sites, where it undergoes proteasomal degradation during infection.

Authors:  Jenny Q Dai-Ju; Ling Li; Lisa A Johnson; Rozanne M Sandri-Goldin
Journal:  J Virol       Date:  2006-04       Impact factor: 5.103
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