| Literature DB >> 6776201 |
V E Jones, P J Cowley, C Allen, C J Elson.
Abstract
IgG with rheumatoid factor activity has been recovered from immune complexes containing IgM rheumatoid factor. This was done by passing serum from patients with rheumatoid arthritis through an immunoadsorbent column of the F(ab')2 fragment of rabbit anti-human mu chain. The recovered IgM was analysed by radioimmunoassays for IgM and IgG rheumatoid factors before and after sucrose density gradient centrifugation at neutral and acid pH. It is considered that the method may be generally applicable for the isolation of immune complexes containing IgM antibodies.Entities:
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Year: 1980 PMID: 6776201 DOI: 10.1016/0022-1759(80)90176-3
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303