Literature DB >> 6776106

Preliminary crystallographic study of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa.

K A Satyshur, S T Rao, J D Lipscomb, J M Wood.   

Abstract

The enzyme protocatechuate 3,4-dioxygenase (EC 1.13.11.3) from Pseudomonas aeruginosa consisting of eight identical subunits (total Mr = 783,100) crystallizes in the monoclinic space group 12 with unit cell dimensions a = 204, b = 129, c = 137 A, and beta = 97.5 degrees. The measured density is 1.26 g ml-1. The Matthews coefficient is 2.27 A3/dalton. The large rod-like crystals diffract to about 2.5 A resolution. The asymmetric unit contains one-half of the enzyme and the enzyme contains an exact 2-fold axis of symmetry.

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Year:  1980        PMID: 6776106

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  2 in total

1.  DNA sequence of the Acinetobacter calcoaceticus catechol 1,2-dioxygenase I structural gene catA: evidence for evolutionary divergence of intradiol dioxygenases by acquisition of DNA sequence repetitions.

Authors:  E L Neidle; C Hartnett; S Bonitz; L N Ornston
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

2.  Purification and characterization of protocatechuate 2,3-dioxygenase from Bacillus macerans: a new extradiol catecholic dioxygenase.

Authors:  S A Wolgel; J E Dege; P E Perkins-Olson; C H Jaurez-Garcia; R L Crawford; E Münck; J D Lipscomb
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

  2 in total

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