Literature DB >> 6772575

Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum.

D A Sack, P K Neogi, M K Alam.   

Abstract

We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.

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Year:  1980        PMID： 6772575      PMCID： PMC551109          DOI： 10.1128/iai.29.1.281-283.1980

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  6 in total

1.  A sensitive method for specific quantitation of secretory IgA.

Authors:  A S Akerlund; L A Hanson; S Ahlstedt; B Carlsson
Journal:  Scand J Immunol       Date:  1977       Impact factor: 3.487

2.  Studies on the quantitation of immunoglobulin in human intestinal secretions.

Authors:  R R Samson; D B McClelland; D J Shearman
Journal:  Gut       Date:  1973-08       Impact factor: 23.059

3.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

4.  Enzyme-linked immunosorbent assays for cholera serology.

Authors:  J Holmgren; A M Svennerholm
Journal:  Infect Immun       Date:  1973-05       Impact factor: 3.441

5.  Automated immunochemical procedures for measurement of immunoglobulins IgG, IgA, and IgM in human serum.

Authors:  L M Killingsworth; J Savory
Journal:  Clin Chem       Date:  1971-09       Impact factor: 8.327

6.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.

Authors:  C B Laurell
Journal:  Anal Biochem       Date:  1966-04       Impact factor: 3.365
  6 in total
  9 in total

1.  Characterization of Aeromonas caviae antigens which cross-react with Shigella boydii 5.

Authors:  M J Albert; F Qadri; M Ansaruzzaman; A K Kibriya; K Haider; P K Neogi; K Alam; A N Alam
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

2.  Prospective study of systemic and mucosal immune responses in dysenteric patients to specific Shigella invasion plasmid antigens and lipopolysaccharides.

Authors:  R A Oberhelman; D J Kopecko; E Salazar-Lindo; E Gotuzzo; J M Buysse; M M Venkatesan; A Yi; C Fernandez-Prada; M Guzman; R León-Barúa
Journal:  Infect Immun       Date:  1991-07       Impact factor: 3.441

3.  Saliva, breast milk, and serum antibody responses as indirect measures of intestinal immunity after oral cholera vaccination or natural disease.

Authors:  M Jertborn; A M Svennerholm; J Holmgren
Journal:  J Clin Microbiol       Date:  1986-08       Impact factor: 5.948

4.  A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin A in serum.

Authors:  M C Booth; J P Gosling; P F Fottrell; C F McCarthy
Journal:  Ir J Med Sci       Date:  1985-04       Impact factor: 1.568

5.  Local and systemic antibody responses and immunological memory in humans after immunization with cholera B subunit by different routes.

Authors:  A M Svennerholm; L Gothefors; D A Sack; P K Bardhan; J Holmgren
Journal:  Bull World Health Organ       Date:  1984       Impact factor: 9.408

Review 6.  Measurement of soluble proteins in lung secretions.

Authors:  R A Stockley
Journal:  Thorax       Date:  1984-04       Impact factor: 9.139

7.  Determination of immunoglobulin A in saliva by immunobead enzyme-linked immunosorbent assay: comparison with single radial immunodiffusion.

Authors:  D Bratthall; R P Ellen
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

8.  Systemic and intestinal immunities after natural typhoid infection.

Authors:  S Sarasombath; N Banchuin; T Sukosol; B Rungpitarangsi; S Manasatit
Journal:  J Clin Microbiol       Date:  1987-06       Impact factor: 5.948

9.  Nonimmunoglobulin fraction of human milk inhibits bacterial adhesion (hemagglutination) and enterotoxin binding of Escherichia coli and Vibrio cholerae.

Authors:  J Holmgren; A M Svennerholm; C Ahrén
Journal:  Infect Immun       Date:  1981-07       Impact factor: 3.441
  9 in total

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