Prolonged ABP synthesis by Sertoli cells cultured in defined medium.

Sertoli cells from 20 day old rats are stimulated to secrete ABP in cultured by a number of hormones and Vitamin A. Maximum ABP secretion in prolonged serum-free culture is seen when FSH, Testosterone, Insulin and Retinol are added to the medium. The plating conditions and type of medium used also affect the total ABP secretion. The decline of ABP secreted into medium by hormone stimulated cultures in prolonged incubations correlates with the detachment of the cells from the culture flask.