Literature DB >> 6771058

Immunonephelometric assay of human apolipoprotein AI.

M F Lopes-Virella, G Virella, G Evans, S B Malenkos, J A Colwell.   

Abstract

We report an immunonephelometric assay for apolipoprotein AI, which involves use of a laser nephelometer and an antiserum prepared in our laboratory. Coefficients of variation were 2.5% within-run, 6.3% between-run. Several types of manipulation of the samples (heating, delipidation, dissociation with urea), reportedly necessary for accurate determinations by radioimmunoassay or gel diffusion immunoassay, did not substantially affect results obtained with our technique. The assay is sensitive, reproducible, and simple, and appears suitable for routine use.

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Year:  1980        PMID： 6771058

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  5 in total

1.  Inherited susceptibility determines the distribution of dense low-density lipoprotein subfraction profiles in familial combined hyperlipidemia.

Authors:  S J Bredie; L A Kiemeney; A F de Haan; P N Demacker; A F Stalenhoef
Journal:  Am J Hum Genet       Date:  1996-04       Impact factor: 11.025

2.  Surface binding, internalization and degradation by cultured human fibroblasts of low density lipoproteins isolated from type 1 (insulin-dependent) diabetic patients: changes with metabolic control.

Authors:  M F Lopes-Virella; G K Sherer; A M Lees; H Wohltmann; R Mayfield; J Sagel; E C LeRoy; J A Colwell
Journal:  Diabetologia       Date:  1982-06       Impact factor: 10.122

3.  A common genetic mechanism determines plasma apolipoprotein B levels and dense LDL subfraction distribution in familial combined hyperlipidemia.

Authors:  S H Juo; S J Bredie; L A Kiemeney; P N Demacker; A F Stalenhoef
Journal:  Am J Hum Genet       Date:  1998-08       Impact factor: 11.025

4.  Metabolism of very low- and low-density lipoproteins isolated from normolipidaemic type 2 (non-insulin-dependent) diabetic patients by human monocyte-derived macrophages.

Authors:  R L Klein; T J Lyons; M F Lopes-Virella
Journal:  Diabetologia       Date:  1990-05       Impact factor: 10.122

5.  Altered inflammation, paraoxonase-1 activity and HDL physicochemical properties in obese humans with and without Prader-Willi syndrome.

Authors:  Gianna Ferretti; Tiziana Bacchetti; Simona Masciangelo; Graziano Grugni; Virginia Bicchiega
Journal:  Dis Model Mech       Date:  2012-07-19       Impact factor: 5.758
  5 in total

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