Literature DB >> 677100

A simple assay technique for pancreatic lipase.

R E Connon, H Van Kley, W A Knight.   

Abstract

A simple and inexpensive procedure is described to include pancreatic lipase in the protocol for evaluation of pancreatic function by duodenal aspiration. The method involves dilution with an albumin solution, a 2-hr incubation with a commercially available substrate mixture, and manual titration with a burette. Results comparable to assay with a pH-stat were obtained. Assay for pancreatic lipase is recommended as a part of the diagnostic procedure of duodenal aspiration.

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Year:  1978        PMID: 677100     DOI: 10.1007/BF01072932

Source DB:  PubMed          Journal:  Am J Dig Dis        ISSN: 0002-9211


  16 in total

1.  CONGENITAL PANCREATIC LIPASE DEFICIENCY.

Authors:  W SHELDON
Journal:  Arch Dis Child       Date:  1964-06       Impact factor: 3.791

2.  Evaluation of pancreatic function tests in confirmed pancreatic disease.

Authors:  W J HENKE; J B VACCA; G E VAN GOIDSENHOVEN; W A KNIGHT
Journal:  Gastroenterology       Date:  1961-09       Impact factor: 22.682

3.  A suitable substrate for the determination of pancreatic lipase in serum and other body fluids.

Authors:  E MacLAY
Journal:  Am J Med Technol       Date:  1948-07

Review 4.  Testing pancreatic function.

Authors:  F P Brooks
Journal:  N Engl J Med       Date:  1972-02-10       Impact factor: 91.245

5.  Deficiency of lipolytic pancreatic enzymes in an adult man.

Authors:  B Arnesjö; I Ihse; H Odeberg; E Stahl
Journal:  Digestion       Date:  1974       Impact factor: 3.216

6.  A model of pancreatic lipase and the orientation of enzymes at interfaces.

Authors:  H Brockerhoff
Journal:  Chem Phys Lipids       Date:  1973-04       Impact factor: 3.329

7.  On the function of bile salts and proteins as cofactors of lipase.

Authors:  H Brockerhoff
Journal:  J Biol Chem       Date:  1971-09-25       Impact factor: 5.157

8.  The output of protein after pancreozymin. A test of pancreatic function.

Authors:  D H Hanscom; B M Jacobson; A Littman
Journal:  Ann Intern Med       Date:  1967-04       Impact factor: 25.391

9.  Serum lipase determination in acute pancreatitis. Clinical appraisal of a new method.

Authors:  H H Patt; S P Kramer; G Woel; D Zeitung; A M Seligman
Journal:  Arch Surg       Date:  1966-05

10.  Fluorometric serum lipase assay: evaluation of monodecanoyl fluorescein as substrate.

Authors:  B Klein; F Standaert
Journal:  Clin Chem       Date:  1975-09       Impact factor: 8.327

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