Use of modified New York City medium for growth of Mycoplasma pneumoniae. A preliminary report.

The recovery of Mycoplasma pneumoniae from clinical specimens is a time-consuming process that requires highly specialized media. Modified New York City (NYC) medium was evaluated for its ability to support the growth of M. pneumoniae, as compared with conventional culture technics for mycoplasma. In this study, 95 stock strains of M. pneumoniae were inoculated onto modified NYC medium, PPLO methylene blue agar, biphasic medium with methylene blue, and PPLO glucose broth with phenol red to compare each medium's growth-supporting properties. The results demonstrated that the modified NYC formulation was a suitable medium for supporting this growth of stock strains of M. pneumoniae and that growth can be detected a minimum of five to six days sooner than by conventional methods.