| Literature DB >> 6768792 |
J A Carnegie, M E McCully, H A Robertson.
Abstract
The antigenic activity of a labile protein postulated to the hormone ovine chorionic somatomammotropin (OcS) is preserved in tissue fixed in either glutaraldehyde or paraformaldehyde and low temperature-embedded in the water-soluble plastic glycol methacrylate. Immunofluorescence techniques used on 1--2 micrometer thick sections show that this protein is located in the cytoplasm of certain fetal chorionic cells in association with small spherical bodies, which may be lipid. The use of borohydrate reduction or treatment with Schiff's reagent to reduce glutaraldehyde-induced background fluorescence is described.Entities:
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Year: 1980 PMID： 6768792 DOI： 10.1177/28.4.6768792
Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479