Literature DB >> 6768700

Cytochemistry of the microtrabecular network in compact nucleoli of hepatocytes treated with cycloheximide.

K Smetana, I Daskal, H Busch.   

Abstract

Compact nucleoli without the segregation of nucleolar components were produced in hepatocytes by the treatment of experimental rats with cycloheximide to facilitate a cytochemical study on the organization of nucleolar components in such nucleoli. The extraction of pepsin pretreated specimens with nucleases (deoxyribonuclease and ribonuclease) demonstrated that compact nucleoli are characterized by a relatively uniform distribution of RNP components which mask a microtrabecular intranucleolar network. This network apparently consists of proteins and contains fine DNA filaments.

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Year:  1980        PMID: 6768700     DOI: 10.1007/bf00493179

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  16 in total

1.  PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.

Authors:  H H MOLLENHAUER
Journal:  Stain Technol       Date:  1964-03

2.  Ultrastructural studies on nucleic acids of nucleolar granular components in Novikoff hepatoma cells.

Authors:  K Smetana; T Unuma; H Busch
Journal:  Exp Cell Res       Date:  1968-07       Impact factor: 3.905

3.  The fine structure of a nucleolar constituent.

Authors:  L Recher; J Whitescarver; L Briggs
Journal:  J Ultrastruct Res       Date:  1969-10

4.  Mechanism of nucleolar segregation--differences in effects of actinomycin D and cycloheximide on nucleoli of rat liver cells.

Authors:  T Unuma; R Senda; M Muramatsu
Journal:  J Electron Microsc (Tokyo)       Date:  1973

5.  Selective removal of the perinucleolar nucleolus-associated chromatin from the isolated nucleoli of livers of thioacetamide-treated rats.

Authors:  T Unuma; L R Floyd; H Busch
Journal:  Exp Cell Res       Date:  1968-09       Impact factor: 3.905

6.  [Ultrastructural demonstration of deoxyribonuclease-sensitive elements in fibrillar zones of the nucleoli of L 929 cell interphasic nuclei].

Authors:  A Anteunis; M Pouchelet; A Gansmüller; R Robineaux
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1975-09-29

7.  Cytological identification and characterization of the nuclear matrix.

Authors:  S Ghosh; N Paweletz; I Ghosh
Journal:  Exp Cell Res       Date:  1978-02       Impact factor: 3.905

8.  [Fibrillary centers of the nucleoli of Ehrlich's tumor cells].

Authors:  G Goessens
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1973-07-16

9.  A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.

Authors:  J H VENABLE; R COGGESHALL
Journal:  J Cell Biol       Date:  1965-05       Impact factor: 10.539

10.  Nuclear matrix. Isolation and characterization of a framework structure from rat liver nuclei.

Authors:  R Berezney; D S Coffey
Journal:  J Cell Biol       Date:  1977-06       Impact factor: 10.539

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  2 in total

1.  Evidence for the existence of a nucleolar skeleton attached to the pore complex-lamina in human fibroblasts.

Authors:  C A Bourgeois; D Bouvier; A P Seve; J Hubert
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

Review 2.  The nuclear skeleton and the spatial arrangement of chromosomes in the interphase nucleus of vertebrate somatic cells.

Authors:  J Hubert; C A Bourgeois
Journal:  Hum Genet       Date:  1986-09       Impact factor: 4.132

  2 in total

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