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Literature DB >> 6765739

Uptake and release of choline in cultures of human glioma cells.

Abstract

Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 microM; Vmax = 56 pmol/min/10(6) cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.

Entities: Chemical Disease Species

Mesh：

Substances：
Choline

Year: 1981 PMID： 6765739 DOI： 10.1007/bf00716273

Source DB: PubMed Journal: Cell Mol Neurobiol ISSN： 0272-4340 Impact factor: 5.046

23 in total

4. Interrelationships among the levels of ATP, adenosine and cyclic AMP in incubated slices of guinea-pig cerebral cortex: effects of depolarizing agents, psychotropic drugs and metabolic inhibitors.

Authors: M Huang; J W Daly
Journal: J Neurochem Date: 1974-08 Impact factor: 5.372

Uptake and release of choline in cultures of human glioma cells.

1. An improved nutrient solution for diploid Chinese hamster and human cell lines.

2. Density-dependent inhibition of 2-deoxy-D-glucose uptake into glioma and neuroblastoma cells in culture.

Review 3. Amino acid transport in isolated neurons and glia.

4. Interrelationships among the levels of ATP, adenosine and cyclic AMP in incubated slices of guinea-pig cerebral cortex: effects of depolarizing agents, psychotropic drugs and metabolic inhibitors.

5. Long term culture of normal and neoplastic human glia.

6. Screening for neurotransmitters: a rapid radiochemical procedure.

7. Transport of neurotransmitter precursors into cultured cells.

8. Morphological alterations and increase of S-100 protein in cultured human glioma cells deprived of serum.

9. Counter transport of glutamine and choline in cultures of human glioma cells.

10. Plaque formation and isolation of pure lines with poliomyelitis viruses.

1. Growth and morphology of neuronal cell lines cultured in perfusion.