Assessment of some genetic non-MHC differences with GVHR-induced anti-listeria activity of macrophages.

Parental cells A injected into (A X B)F1 heterozygotes induce a graft-versus-host reaction (GVHR) which induces systemic activation of anti-Listeria-bactericidal capacity of host macrophages. When donor lymphocytes differ from parent A with respect to various non-H-2 genetic markers, they may or may not be able to induce a GVHR. Some, but not all, known and some unknown non-H-2 differences can be assessed by this method within nine to 12 days. The method is described and some of the following non-H-2 differences are shown to influence GVHR-induced macrophage activation: male H-Y antigen, H-3 or H-4 (but not H-1 or H-9), and as yet underlined differences that apparently exist between mouse substrains of the same or similar designation, but obtained from different breeding establishments.